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Methylation and cop...
Methylation and copy number profiling : emerging tools to differentiate osteoblastoma from malignant mimics?
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- Ameline, Baptiste (author)
- University Hospital Basel
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- Nathrath, Michaela (author)
- Klinikum Kassel GmbH,Klinikum rechts der Isar
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- Nord, Karolin H. (author)
- Lund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Komplexa kromosomförändringar i cancer,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine,Genetic chaos in aggressive cancer,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments
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- de Flon, Felix Haglund (author)
- Karolinska Institutet,Karolinska Institute,Karolinska University Hospital
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- Bovée, Judith V.M.G. (author)
- Leiden University Medical Centre
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- Krieg, Andreas H. (author)
- University Children's Hospital, Basel
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- Höller, Sylvia (author)
- University Hospital Basel,University of Zurich
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- Hench, Jürgen (author)
- University Hospital Basel
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- Baumhoer, Daniel (author)
- University Hospital Basel
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(creator_code:org_t)
- Elsevier BV, 2022
- 2022
- English.
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In: Modern Pathology. - : Elsevier BV. - 0893-3952. ; 35:9, s. 1204-1211
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Abstract
Subject headings
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- Rearrangements of the transcription factors FOS and FOSB have recently been identified as the genetic driver event underlying osteoid osteoma and osteoblastoma. Nuclear overexpression of FOS and FOSB have since then emerged as a reliable surrogate marker despite limitations in specificity and sensitivity. Indeed, osteosarcoma can infrequently show nuclear FOS expression and a small fraction of osteoblastomas seem to arise independent of FOS/FOSB rearrangements. Acid decalcification and tissue preservation are additional factors that can negatively influence immunohistochemical testing and make diagnostic decision-making challenging in individual cases. Particularly aggressive appearing osteoblastomas, also referred to as epithelioid osteoblastomas, and osteoblastoma-like osteosarcoma can be difficult to distinguish, underlining the need for additional markers to support the diagnosis. Methylation and copy number profiling, a technique well established for the classification of brain tumors, might fill this gap. Here, we set out to comprehensively characterize a series of 77 osteoblastomas by immunohistochemistry, fluorescence in-situ hybridization as well as copy number and methylation profiling and compared our findings to histologic mimics. Our results show that osteoblastomas are uniformly characterized by flat copy number profiles that can add certainty in reaching the correct diagnosis. The methylation cluster formed by osteoblastomas, however, so far lacks specificity and can be misleading in individual cases.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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