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Secretion of iron(I...
Secretion of iron(III)-reducing metabolites during protein acquisition by the ectomycorrhizal fungus paxillus involutus
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- Shah, Firoz (författare)
- Lund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Mikrobiologisk ekologi,Forskargrupper vid Lunds universitet,Department of Biology,Faculty of Science,Microbial Ecology,Lund University Research Groups,Lund University, Lund, Sweden
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- Gressler, Markus (författare)
- Friedrich Schiller University Jena
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- Nehzati, Susan (författare)
- Lund University,Lunds universitet,MAX IV-laboratoriet,Mikrobiologisk ekologi,Forskargrupper vid Lunds universitet,MAX IV Laboratory,Microbial Ecology,Lund University Research Groups
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- De Beeck, Michiel Op (författare)
- Lund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Mikrobiologisk ekologi,Forskargrupper vid Lunds universitet,Department of Biology,Faculty of Science,Microbial Ecology,Lund University Research Groups
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- Gentile, Luigi (författare)
- Lund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Mikrobiologisk ekologi,Forskargrupper vid Lunds universitet,Department of Biology,Faculty of Science,Microbial Ecology,Lund University Research Groups
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- Hoffmeister, Dirk (författare)
- Friedrich Schiller University Jena
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- Persson, Per (författare)
- Lund University,Lunds universitet,Centrum för miljö- och klimatvetenskap (CEC),Naturvetenskapliga fakulteten,Centre for Environmental and Climate Science (CEC),Faculty of Science
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- Tunlid, Anders (författare)
- Lund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Mikrobiologisk ekologi,Forskargrupper vid Lunds universitet,Department of Biology,Faculty of Science,Microbial Ecology,Lund University Research Groups
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(creator_code:org_t)
- 2020-12-24
- 2021
- Engelska 16 s.
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Ingår i: Microorganisms. - : MDPI AG. - 2076-2607. ; 9:1
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://www.mdpi.com...
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https://lup.lub.lu.s...
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https://doi.org/10.3...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The ectomycorrhizal fungus Paxillus involutus decomposes proteins using a two-step mechanism, including oxidation and proteolysis. Oxidation involves the action of extracellular hydroxyl radicals (•OH) generated by the Fenton reaction. This reaction requires the presence of iron(II). Here, we monitored the speciation of extracellular iron and the secretion of iron(III)-reducing metabolites during the decomposition of proteins by P. involutus. X-ray absorption spectroscopy showed that extracellular iron was mainly present as solid iron(III) phosphates and oxides. Within 1 to 2 days, these compounds were reductively dissolved, and iron(II) complexes were formed, which remained in the medium throughout the incubation. HPLC and mass spectrometry detected five extracellular iron(III)-reducing metabolites. Four of them were also secreted when the fungus grew on a medium containing ammonium as the sole nitrogen source. NMR identified the unique iron(III)-reductant as the diarylcyclopentenone involutin. Involutin was produced from day 2, just before the elevated •OH production, preceding the oxidation of BSA. The other, not yet fully characterized iron(III)-reductants likely participate in the rapid reduction and dissolution of solid iron(III) complexes observed on day one. The production of these metabolites is induced by other environmental cues than for involutin, suggesting that they play a role beyond the Fenton chemistry associated with protein oxidation.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- Ectomycorrhizal fungi
- Fenton reaction
- Involutin
- Iron reduction
- Secondary metabolites
- Virology
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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