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Cell-specific proce...
Cell-specific processing of chromogranin A in endocrine cells of the rat stomach
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- Norlén, Per (författare)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine
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Curry, William J. (författare)
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- Björkqvist, Maria (författare)
- Lund University,Lunds universitet,Translationell neuroendokrinologi,Forskargrupper vid Lunds universitet,Translational Neuroendocrinology,Lund University Research Groups
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Maule, Aron (författare)
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Cunningham, Rodat T. (författare)
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Hogg, Robert B. (författare)
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Harriott, Pat (författare)
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Johnston, Colin F. (författare)
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Hutton, John C. (författare)
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- Håkanson, Rolf (författare)
- Lund University,Lunds universitet,Drug Target Discovery,Forskargrupper vid Lunds universitet,Lund University Research Groups
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(creator_code:org_t)
- 2001
- 2001
- Engelska.
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Ingår i: Journal of Histochemistry and Cytochemistry. - 0022-1554. ; 49:1, s. 41535-41535
- Relaterad länk:
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http://www.jhc.org/c...
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Abstract
Ämnesord
Stäng
- The rat stomach is rich in endocrine cells. The acid-producing (oxyntic) mucosa contains ECL cells, A-like cells, and somatostatin (D) cells, and the antrum harbours gastrin (G) cells, enterochromaffin (EC) cells and D cells. Although chromogranin A (CgA) occurs in all these cells, its processing appears to differ from one cell type to another. Eleven antisera generated to different regions of rat CgA, two antisera generated to a human (h) CgA sequences, and one to a bovine Ib) CgA sequence, respectively, were employed together with antisera directed towards cell-specific markers such as gastrin (G cells), serotonin (EC cells), histidine decarboxylsae (ECL cells) and somatostatin (D cells) to characterize the expression of CgA and CgA-derived peptides in the various endocrine cell populations of the rat stomach. In the oxyntic mucosa, antisera raised against CgA(291-319) and CGA(316-321) immunostained D cells exclusively, whereas antisera raised against bCgA(82-91) and CgA(121-128) immunostained A-like cells and D cells. Antisera raised against CgA(318-349) and CgA(437-448) immunostained ECL cells and A-like cells, but not D cells. In the antrum, antisera against CgA(291-319) immunostained D cells, and antisera against CgA(351-356) immunostained G cells. Our observations suggest that each individual endocrine cell type in the rat stomach generates a unique mixture of CgA-derived peptides, probably reflecting cell-specific differences in the post-translational processing of CgA and its peptide products. A panel of antisera that recognize specific domains of CgA may help to identify individual endocrine cell populations.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Nyckelord
- chromogranin A
- ECL cell
- D cell
- A-like cell
- EC cell
- G cell
- processing
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Norlén, Per
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Curry, William J ...
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Björkqvist, Mari ...
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Maule, Aron
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Cunningham, Roda ...
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Hogg, Robert B.
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visa fler...
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Harriott, Pat
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Johnston, Colin ...
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Hutton, John C.
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Håkanson, Rolf
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visa färre...
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