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A Murine Mycobacter...
A Murine Mycobacterium marinum Infection Model for Longitudinal Analyses of Disease Development and the Inflammatory Response
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- Lienard, Julia (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Mikrobiologigruppen,Forskargrupper vid Lunds universitet,Functional zoology,Department of Biology,Faculty of Science,Microbiology Group,Lund University Research Groups
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- Munke, Kristina (författare)
- Lund University,Lunds universitet,Slemhinnans immunologi,Forskargrupper vid Lunds universitet,Mucosal Immunology,Lund University Research Groups
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- Carlsson, Fredric (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Mikrobiologigruppen,Forskargrupper vid Lunds universitet,Functional zoology,Department of Biology,Faculty of Science,Microbiology Group,Lund University Research Groups
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Nordenfelt, Pontus (redaktör/utgivare)
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Collin, Mattias (redaktör/utgivare)
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(creator_code:org_t)
- 2
- 2023
- 2023
- Engelska 14 s.
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Ingår i: Bacterial Pathogenesis : Methods and Protocols - Methods and Protocols. - 1064-3745 .- 1940-6029. - 9781071632437 - 9781071632451 ; 2674, s. 313-326
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Mycobacterial infections, including tuberculosis, are a major health problem globally. Prevention and treatments of tuberculosis are challenging due to the poor efficacy of the current vaccine and the emergence of drug-resistant strains. Therefore, it is critical to increase our basic understanding of mycobacterial virulence strategies as well as the host immune response during infection in the complex in vivo setting. While existing infection models provide valuable tools for investigating mycobacterial pathogenesis, they also exhibit limitations that can be addressed by the development of complementary models. Here we describe recent advances to the murine Mycobacterium marinum infection model, in which the bacteria produce a local infection restricted to the tail tissue. The M. marinum model has the advantage of mimicking some of the key hallmarks of human tuberculosis not replicated in the conventional murine Mycobacterium tuberculosis model, such as the formation of granulomas with central caseating necrosis and the spontaneous development of a latency-like stage. Moreover, the model is non-lethal and enables longitudinal analysis of disease development in live animals. In this chapter, we report protocols to prepare infected tissue samples for detailed and quantitative analysis of the immune response by flow cytometry, immunofluorescence microscopy, RT-qPCR, ELISA, and Western blot, as well as for the analysis of bacterial load and localization.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Nyckelord
- Bacterial localization
- Caseating necrosis
- Granuloma formation
- Inflammatory response
- Latency
- Mouse model
- Mycobacterium marinum
- Tuberculosis
Publikations- och innehållstyp
- kap (ämneskategori)
- ref (ämneskategori)
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