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- Duan, Rui-DongLund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Gastro,Forskargrupper vid Lunds universitet,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University Research Groups (author)
Is there a specific lysophospholipase in human pancreatic juice?
- Article/chapterEnglish1993
Publisher, publication year, extent ...
- Elsevier BV,1993
- 5 s.
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- LIBRIS-ID:oai:lup.lub.lu.se:1454023c-8c86-4140-a875-32c2f9b48831
- https://lup.lub.lu.se/record/1454023c-8c86-4140-a875-32c2f9b48831URI
- https://doi.org/10.1016/0005-2760(93)90236-3DOI
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- Language:English
- Summary in:English
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- The existence of a specific lysophospholipase in human pancreatic juice was evaluated. The proteins were separated by a series of chromatographic steps including Sephacryl S-200, cholate-Sepharose 4B, Sephadex G-100 and CM-Sephadex G-50. The enzyme activities against 1-palmitoyl lysolecithin (LL) as well as tributyrin (TB) and p-nitrophenyl butyrate (PNPB) were determined in all the fractions of these purification procedures. Enzyme activity against LL was always eluted in parallel with activities against TB and PNPB, and no unique activity against LL could be found. The specific activity against LL was 40-times lower than that against PNPB and 200-times lower than that against TB. It is concluded that there is no unique lysophospholipase in human pancreatic juice and that the hydrolysis of lysolecithin is most likely performed by carboxyl ester lipase.
Subject headings and genre
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Läkemedelskemi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Medicinal Chemistry hsv//eng
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- Borgström, BengtLund University (author)
- Medicin, LundSektion II (creator_code:org_t)
Related titles
- In:Biochimica et Biophysica Acta: Elsevier BV1167:3, s. 326-3300006-3002
- In:Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism: Elsevier BV1167:3, s. 326-3300005-2760
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