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Age-associated diff...
Age-associated differences in the human lung extracellular matrix
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- Ngassie, Maunick Lefin Koloko (författare)
- University Medical Center Groningen
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- De Vries, Maaike (författare)
- University Medical Center Groningen
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- Borghuis, Theo (författare)
- University Medical Center Groningen
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- Timens, Wim (författare)
- University Medical Center Groningen
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- Sin, Don D. (författare)
- University of British Columbia
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- Nickle, David (författare)
- Monoceros Bio
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- Joubert, Philippe (författare)
- Institut universitaire de cardiologie et de pneumologie de Québec
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- Horvatovich, Peter (författare)
- University of Groningen
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- Marko-Varga, György (författare)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Clinical Protein Science and Imaging,Forskargrupper vid Lunds universitet,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Lund University Research Groups
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- Teske, Jacob J. (författare)
- Mayo Clinic Minnesota
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- Vonk, Judith M. (författare)
- University Medical Center Groningen
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- Gosens, Reinoud (författare)
- University of Groningen,University Medical Center Groningen
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- Prakash, Y. S. (författare)
- Mayo Clinic Minnesota
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- Burgess, Janette K. (författare)
- University Medical Center Groningen
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- Brandsma, Corry Anke (författare)
- University Medical Center Groningen
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(creator_code:org_t)
- 2023
- 2023
- Engelska.
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Ingår i: American Journal of Physiology - Lung Cellular and Molecular Physiology. - 1040-0605. ; 324:5, s. 799-814
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Extracellular matrix (ECM) remodeling has been associated with chronic lung diseases. However, information about specific age-associated differences in lung ECM is currently limited. In this study, we aimed to identify and localize age-associated ECM differences in human lungs using comprehensive transcriptomic, proteomic, and immunohistochemical analyses. Our previously identified age-associated gene expression signature of the lung was re-analyzed limiting it to an aging signature based on 270 control patients (37–80 years) and focused on the Matrisome core geneset using geneset enrichment analysis. To validate the age-associated transcriptomic differences on protein level, we compared the age-associated ECM genes (false discovery rate, FDR < 0.05) with a profile of age-associated proteins identified from a lung tissue proteomics dataset from nine control patients (49–76 years) (FDR < 0.05). Extensive immunohistochemical analysis was used to localize and semi-quantify the age-associated ECM differences in lung tissues from 62 control patients (18–82 years). Comparative analysis of transcriptomic and proteomic data identified seven ECM proteins with higher expression with age at both gene and protein levels: COL1A1, COL6A1, COL6A2, COL14A1, FBLN2, LTBP4, and LUM. With immunohistochemistry, we demonstrated higher protein levels with age for COL6A2 in whole tissue, parenchyma, airway wall, and blood vessel, for COL14A1 and LUM in bronchial epithelium, and COL1A1 in lung parenchyma. Our study revealed that higher age is associated with lung ECM remodeling, with specific differences occurring in defined regions within the lung. These differences may affect lung structure and physiology with aging and as such may increase susceptibility to developing chronic lung diseases.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Lungmedicin och allergi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Respiratory Medicine and Allergy (hsv//eng)
Nyckelord
- aging
- airway wall
- extracellular matrix
- lung
- parenchyma
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Ngassie, Maunick ...
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De Vries, Maaike
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Borghuis, Theo
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Timens, Wim
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Sin, Don D.
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Nickle, David
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visa fler...
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Joubert, Philipp ...
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Horvatovich, Pet ...
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Marko-Varga, Gyö ...
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Teske, Jacob J.
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Vonk, Judith M.
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Gosens, Reinoud
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Prakash, Y. S.
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Burgess, Janette ...
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Brandsma, Corry ...
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visa färre...
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Lunds universitet