Sökning: WFRF:(Jemaà Mohamed) >
Inhibition of colon...
Inhibition of colon carcinoma cell migration following treatment with purified venom from lesser weever fish (Trachinus Vipera)
-
- Fezai, Myriam (författare)
- University of Carthage,University of Tübingen
-
- Slaymi, Chaker (författare)
- University of Montpellier
-
- Ben-Attia, Mossadok (författare)
- University of Carthage
-
visa fler...
-
- Kroemer, Guido (författare)
- Hôpital Européen Georges-Pompidou,Paris-Sorbonne University,Paris Descartes University,Institut Gustave Roussy,Karolinska University Hospital
-
- Lang, Florian (författare)
- University of Tübingen,Heinrich Heine University Düsseldorf
-
- Jemaà, Mohamed (författare)
- Lund University,Lunds universitet,Avdelningen för translationell cancerforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Translational Cancer Research,Department of Laboratory Medicine,Faculty of Medicine,University of Tübingen
-
visa färre...
-
(creator_code:org_t)
- 2017-04-26
- 2017
- Engelska 10 s.
-
Ingår i: Cellular Physiology and Biochemistry. - : S. Karger AG. - 1015-8987 .- 1421-9778. ; 41:6, s. 2279-2288
- Relaterad länk:
-
http://dx.doi.org/10... (free)
-
visa fler...
-
https://www.karger.c...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background: Injury by the sting of Lesser weever fish (Trachinus vipera) may lead to severe pain, edema or tissue necrosis. Cellular effects of the venom are still incompletely understood. Previous observations revealed that purified Lesser weever fish venom (LWFV) induces suicidal death of erythrocytes and HCT116 human colon carcinoma cells. The present study addressed the effect of the venom on colon carcinoma cell toxicity, shape and migration both in p53+/+ and/or p53-/- conditions. Methods: Cells were exposed to medium without or with 500 μg/ ml LWFV. Cell shape, cell area and circularity were visualized and quantified by fluorescence microscopy. Cell volume, granularity and cells toxicity were assessed via the apoptotic parameters dissipation of mitochondrial inner transmembrane potential, phosphatidylserine surface exposure and cell membrane permeabilization were measured utilizing flow cytometry. Cell migration was evaluated using wound healing assay and two-dimensional migration assay. Results: LWFV treatment was followed by a marked change of cell shape and size, significant decrease of cell area and circularity, significant impairment of cell migration, as well as induction of apoptosis after long exposition. Conclusions: LWFV exposure leads to cell shrinkage, increased granularity, apoptosis and impairment of cell migration, effects presumably contributing to LWFV-induced tissue injury.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- Cell volume
- Granularity
- Lesser weever fish venom
- Migration
- Mitochondrial pathway of apoptosis
- p53
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas