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  • Stsiapanava, AlenaKarolinska Institutet (author)

Product formation controlled by substrate dynamics in leukotriene A4 hydrolase.

  • Article/chapterEnglish2014

Publisher, publication year, extent ...

  • Elsevier BV,2014

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:1c1173d0-8c76-48f0-a356-afdef68e8497
  • https://lup.lub.lu.se/record/4223819URI
  • https://doi.org/10.1016/j.bbapap.2013.12.003DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:128192159URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Leukotriene A4 hydrolase/aminopeptidase (LTA4H) (EC 3.3.2.6) is a bifunctional zinc metalloenzyme with both an epoxide hydrolase and an aminopeptidase activity. LTA4H from the African claw toad, Xenopus laevis (xlLTA4H) has been shown to, unlike the human enzyme, convert LTA4 to two enzymatic metabolites, LTB4 and another biologically active product Δ(6)-trans-Δ(8)-cis-LTB4 (5(S),12R-dihydroxy-6,10-trans-8,14-cis-eicosatetraenoic acid). In order to study the molecular aspect of the formation of this product we have characterized the structure and function of xlLTA4H. We solved the structure of xlLTA4H to a resolution of 2.3Å. It is a dimeric structure where each monomer has three domains with the active site in between the domains, similar as to the human structure. An important difference between the human and amphibian enzyme is the phenylalanine to tyrosine exchange at position 375. Our studies show that mutating F375 in xlLTA4H to tyrosine abolishes the formation of the LTB4 isomeric product Δ(6)-trans-Δ(8)-cis-LTB4. In an attempt to understand how one amino acid exchange leads to a new product profile as seen in the xlLTA4H, we performed a conformer analysis of the triene part of the substrate LTA4. Our results show that the Boltzmann distribution of substrate conformers correlates with the observed distribution of products. We suggest that the observed difference in product profile between the human and the xlLTA4H arises from different level of discrimination between substrate LTA4 conformers.

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Added entries (persons, corporate bodies, meetings, titles ...)

  • Tholander, FredrikKarolinska Institutet (author)
  • Kumar, Ramakrishnan BKarolinska Institutet (author)
  • Qureshi, Abdul Aziz (author)
  • Niegowski, Damian (author)
  • Hasan, MahmudulLund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)med-mmh (author)
  • Thunnissen, MarjoleinLund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)mbfys-mo (author)
  • Haeggström, Jesper ZKarolinska Institutet (author)
  • Rinaldo-Matthis, AgnesKarolinska Institutet (author)
  • Karolinska InstitutetBiokemi och Strukturbiologi (creator_code:org_t)

Related titles

  • In:Biochimica et Biophysica Acta: Elsevier BV1844:2, s. 439-4460006-3002
  • In:Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics: Elsevier BV1844:2, s. 439-4461570-9639

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