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Expression and dist...
Expression and distribution of key proteins of the endocannabinoid system in the human seminal vesicles
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- Ückert, S. (author)
- Hannover Medical School
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- la Croce, G. (author)
- Vita-Salute San Raffaele University
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- Bettiga, A. (author)
- Vita-Salute San Raffaele University
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- Albrecht, K. (author)
- Hannover Medical School
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- Buono, R. (author)
- University of Southern California
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- Benigni, F. (author)
- Vita-Salute San Raffaele University
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- Kuczyk, M. A. (author)
- Hannover Medical School
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- Hedlund, P. (author)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine
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(creator_code:org_t)
- 2017-08-08
- 2018
- English.
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In: Andrologia. - : Hindawi Limited. - 0303-4569. ; 50:2
- Related links:
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http://dx.doi.org/10...
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Abstract
Subject headings
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- The endocannabinoid system (ECS), comprising the cannabinoid receptors (CBR), their ligands, and enzymes controlling the turnover of endocannabinoids, has been suggested to be involved in male reproductive function. As information is scarce on the expression of the ECS in human male reproductive tissues, this study aimed to investigate by means of molecular biology (RT-PCR) and immunohistochemistry/immunofluorescence the expression and distribution of CB1 and CB2, GPR55 (an orphan G protein-coupled receptor that recognises cannabinoid ligands) and FAAH (isoforms 1 and 2) in the human seminal vesicles (SV). The specimens expressed PCR products corresponding to CB1 (66 bp), CB2 (141 bp), GPR55 (112 bp), FAAH1 (260 bp) and FAAH2 (387 bp). Immumohistochemistry revealed dense expression of CB1, CB2 and GPR55 located to the pseudo-stratified columnar epithelium and varicose nerves (also characterised by the expression of vasoactive intestinal polypeptide and calcitonin gene-related peptide). Cytosolic staining for FAAH1 and FAAH2 was seen in cuboidal cells of all layers of the epithelium. No immunoreactivity was detected in the smooth musculature or nerve fibres. CB1, CB2, GPR55, FAAH1 and FAAH2 are highly expressed in the human SV. Considering their localisation, the ECS may be involved in epithelial homeostasis, secretory function or autonomic mechano-afferent signalling.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Keyword
- endocannabinoid receptors
- fatty acid amide hydrolase
- human seminal vesicles
- immunohistochemistry
- molecular biology
Publication and Content Type
- art (subject category)
- ref (subject category)
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