Sökning: onr:"swepub:oai:lup.lub.lu.se:1fa52ddd-d954-40c1-b6bf-4c6cd1b84d8b" >
Non-ischemic Heart ...
-
Critchley, William R.University of Manchester,Manchester University NHS Foundation Trust
(författare)
Non-ischemic Heart Preservation via Hypothermic Cardioplegic Perfusion Induces Immunodepletion of Donor Hearts Resulting in Diminished Graft Infiltration Following Transplantation
- Artikel/kapitelEngelska2020
Förlag, utgivningsår, omfång ...
-
2020-07-28
-
Frontiers Media SA,2020
Nummerbeteckningar
-
LIBRIS-ID:oai:lup.lub.lu.se:1fa52ddd-d954-40c1-b6bf-4c6cd1b84d8b
-
https://lup.lub.lu.se/record/1fa52ddd-d954-40c1-b6bf-4c6cd1b84d8bURI
-
https://doi.org/10.3389/fimmu.2020.01621DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:art swepub-publicationtype
-
Ämneskategori:ref swepub-contenttype
Anmärkningar
-
Introduction: Many donor organs contain significant leukocyte reservoirs which upon transplantation activate recipient leukocytes to initiate acute rejection. We aimed to assess whether non-ischemic heart preservation via ex vivo perfusion promotes immunodepletion and alters the inflammatory status of the donor organ prior to transplantation. Methods: Isolated porcine hearts underwent ex vivo hypothermic, cardioplegic perfusion for 8 h. Leukocyte populations were quantified in left ventricle samples by flow cytometry. Cell-free DNA, cytokines, and chemokines were quantified in the perfusate. Tissue integrity was profiled by targeted proteomics and a histological assessment was performed. Heterotopic transplants comparing ex vivo hypothermic preservation and static cold storage were utilized to assess graft infiltration as a solid clinical endpoint. Results: Ex vivo perfusion significantly immunodepleted myocardial tissue. The perfusate displayed a selective, pro-inflammatory cytokine/chemokine pattern dominated by IFN-γ. The tissue molecular profile was improved following perfusion by diminished expression of nine pro-apoptotic and six ischemia-associated proteins. Histologically, no evidence of tissue damage was observed and cardiac troponin I was low throughout perfusion. Cell-free DNA was detected, the source of which may be necrotic/apoptotic leukocytes. Post-transplant graft infiltration was markedly reduced in terms of both leucocyte distribution and intensity of foci. Conclusions: These findings demonstrate that ex vivo perfusion significantly reduced donor heart immunogenicity via loss of resident leukocytes. Despite the pro-inflammatory cytokine pattern observed, a pro-survival and reduced ischemia-related profile was observed, indicating an improvement in graft viability by perfusion. Diminished graft infiltration was observed in perfused hearts compared with those preserved by static cold storage following 48 h of transplantation.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Stone, John P.University of Manchester,Manchester University NHS Foundation Trust
(författare)
-
Liao, QiumingLund University,Lunds universitet,Hjärt- och lungtransplantation,Forskargrupper vid Lunds universitet,Heart and Lung transplantation,Lund University Research Groups,Skåne University Hospital(Swepub:lu)thor-qli
(författare)
-
Qin, GuangqiLund University,Lunds universitet,Hjärt- och lungtransplantation,Forskargrupper vid Lunds universitet,Heart and Lung transplantation,Lund University Research Groups,Skåne University Hospital(Swepub:lu)med-ggq
(författare)
-
Risnes, Ivar
(författare)
-
Trafford, AndrewUniversity of Manchester
(författare)
-
Scott, HelgeUniversity of Oslo
(författare)
-
Sjöberg, TrygveLund University,Lunds universitet,Hjärt- och lungtransplantation,Forskargrupper vid Lunds universitet,Heart and Lung transplantation,Lund University Research Groups,Skåne University Hospital(Swepub:lu)thor-tsj
(författare)
-
Steen, StigLund University,Lunds universitet,Hjärt- och lungtransplantation,Forskargrupper vid Lunds universitet,Heart and Lung transplantation,Lund University Research Groups,Skåne University Hospital(Swepub:lu)thor-sst
(författare)
-
Fildes, James E.Manchester University NHS Foundation Trust,University of Manchester
(författare)
-
University of ManchesterManchester University NHS Foundation Trust
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Frontiers in Immunology: Frontiers Media SA111664-3224
Internetlänk
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Critchley, Willi ...
-
Stone, John P.
-
Liao, Qiuming
-
Qin, Guangqi
-
Risnes, Ivar
-
Trafford, Andrew
-
visa fler...
-
Scott, Helge
-
Sjöberg, Trygve
-
Steen, Stig
-
Fildes, James E.
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Kardiologi
- Artiklar i publikationen
-
Frontiers in Imm ...
- Av lärosätet
-
Lunds universitet