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Composition analysi...
Composition analysis and minimal treatments to solubilize polysaccharides from the brown seaweed Laminaria digitata for microbial growth of thermophiles
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- Allahgholi, Leila (author)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Sardari, Roya R.R. (author)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Hakvåg, Sigrid (author)
- Norwegian University of Science and Technology
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- Ara, Kazi Z.G. (author)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Kristjansdottir, Thordis (author)
- Matís ltd. – Icelandic food and biotech R&D
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- Aasen, Inga Marie (author)
- Foundation for Scientific and Industrial Research (SINTEF)
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- Fridjonsson, Olafur H. (author)
- Matís ltd. – Icelandic food and biotech R&D
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- Brautaset, Trygve (author)
- Norwegian University of Science and Technology
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- Hreggvidsson, Gudmundur Oli (author)
- Matís ltd. – Icelandic food and biotech R&D,University of Iceland
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- Karlsson, Eva Nordberg (author)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- 2020-03-30
- 2020
- English 15 s.
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In: Journal of Applied Phycology. - : Springer Science and Business Media LLC. - 0921-8971 .- 1573-5176. ; 32:3, s. 1933-1947
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Abstract
Subject headings
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- Brown macroalgae (Phaeophyta) hold high potential as feedstock for biorefineries due to high biomass productivity and carbohydrate content. They are, however, a challenging, unconventional feedstock for microbial refining and several processing problems need to be solved to make them a viable option. Pre-treatment is necessary to enhance accessibility and solubility of the biomass components but should be minimal and mild to assure sustainable and cost-effective processing. Here, two routes to pre-treatLaminaria digitata to release polysaccharides were investigated: hot water pre-treatment by autoclaving (121 °C, 20 min or 60 min) and a two-step extraction with mild acid (0.1 M HCl) followed by alkaline treatment. Hot water pre-treatment resulted in partial extraction of a mixture of polysaccharides consisting of alginate, fucoidan and laminarin. After mild acid pre-treatment, alginate was found in the remaining insoluble residues and was extracted in a second step via alkaline treatment using Na2CO3 (0.15 M) at 80 °C and CaCl2 (10%) for the precipitation. In addition to carbohydrates, a fraction of other components such as proteins, phenolic compounds, minerals and trace elements was detected in the extracts. Cultivation of the thermophilic bacterial strains Rhodothermus marinus DSM 16675 and Bacillus methanolicus MGA3 (ATCC 53907) in media supplemented with the respective extracts resulted in growth of both strains, indicating that they were able to utilize the available carbon source for growth. R. marinus displayed the highest cell density in the medium containing the extract from acid pre-treatment, whereas B. methanolicus growth was highest with the extract from hot water pre-treatment.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Miljöbioteknik -- Vattenbehandling (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Environmental Biotechnology -- Water Treatment (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik -- Kemiska processer (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering -- Chemical Process Engineering (hsv//eng)
Keyword
- Bacillus methanolicus
- Carbohydrate extraction
- Laminaria digitata
- Mild physicochemical pre-treatment
- Rhodothermus marinus
Publication and Content Type
- art (subject category)
- ref (subject category)
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Allahgholi, Leil ...
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Sardari, Roya R. ...
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Hakvåg, Sigrid
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Ara, Kazi Z.G.
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Kristjansdottir, ...
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Aasen, Inga Mari ...
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Fridjonsson, Ola ...
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Brautaset, Trygv ...
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Hreggvidsson, Gu ...
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Karlsson, Eva No ...
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- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Environmental Bi ...
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and Water Treatment
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Chemical Enginee ...
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and Chemical Process ...
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Lund University