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BDNF downregulates ...
BDNF downregulates 5-HT2A receptor protein levels in hippocampal cultures
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Trajkovska, V. (author)
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Santini, M. A. (author)
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Marcussen, A. B. (author)
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Thomsen, M. S. (author)
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Hansen, H. H. (author)
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Mikkelsen, J. D. (author)
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Arneberg, L. (author)
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- Kokaia, Merab (author)
- Lund University,Lunds universitet,Wallenberg Neurocentrum, Lund,Medicinska fakulteten,Wallenberg Neuroscience Centre, Lund,Faculty of Medicine
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Knudsen, G. M. (author)
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Aznar, S. (author)
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(creator_code:org_t)
- Elsevier BV, 2009
- 2009
- English.
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In: Neurochemistry International. - : Elsevier BV. - 0197-0186. ; 55:7, s. 697-702
- Related links:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Both brain-derived neurotrophic factor (BDNF) and the serotonin receptor 2A (5-HT2A) have been related to depression pathology. Specific 5-HT2A receptor changes seen in BDNF conditional mutant mice suggest that BDNF regulates the 5-HT2A receptor level. Here we show a direct effect of BDNF on 5-HT2A receptor protein levels in primary hippocampal neuronal and mature hippocampal organotypic cultures exposed to different BDNF concentrations for either 1, 3, 5 or 7 days. In vivo effects of BDNF on hippocampal 5-HT2A receptor levels were further corroborated in (BDNF +/-) mice with reduced BDNF levels. In primary neuronal cultures, 7 days exposure to 25 and 50 ng/mL BDNF resulted in downregulation of 5-HT2A, but not of 5-HT1A, receptor protein levels. The BDNF-associated downregulation of 5-HT2A receptor levels was also observed in mature hippocampal organotypic cultures, excluding confounding effects of BDNF on immature tissue. BDNF +/- mice showed significant increased 5-HT2A receptor levels in hippocampus confirming the association between 5-HT2A receptor and BDNF levels in vivo. In conclusion, our results point to a regulatory role of BDNF on 5-HT2A receptor levels. This interaction may be an important mechanism in the role of BDNF in affective disorders emphasizing the need for further elucidating the specificity and the mechanism behind this regulation. (C) 2009 Elsevier Ltd. All rights reserved.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Hippocampus
- Mature organotypic cultures
- Serotonin
- Neurotrophins
- Depression
- Neuronal cultures
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Trajkovska, V.
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Santini, M. A.
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Marcussen, A. B.
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Thomsen, M. S.
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Hansen, H. H.
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Mikkelsen, J. D.
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show more...
-
Arneberg, L.
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Kokaia, Merab
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Knudsen, G. M.
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Aznar, S.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
- Articles in the publication
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Neurochemistry I ...
- By the university
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Lund University