Phosphoproteomic insights into processes influenced by the kinase-like protein DIA1/C3orf58

Hareza, AgnieszkaInternational Institute of Molecular and Cell Biology, Warsaw,Warsaw University of Life Sciences (författare)

Many kinases are still 'orphans,' which means knowledge about their substrates, and often also about the processes they regulate, is lacking. Here, DIA1/C3orf58, a member of a novel predicted kinase-like family, is shown to be present in the endoplasmic reticulum and to influence trafficking via the secretory pathway. Subsequently, DIA1 is subjected to phosphoproteomics analysis to cast light on its signalling pathways. A liquid chromatography-tandem mass spectrometry proteomic approach with phosphopeptide enrichment is applied to membrane fractions of DIA1-overexpressing and control HEK293T cells, and phosphosites dependent on the presence of DIA1 are elucidated. Most of these phosphosites belonged to CK2- and proline-directed kinase types. In parallel, the proteomics of proteins immunoprecipitated with DIA1 reported its probable interactors. This pilot study provides the basis for deeper studies of DIA1 signalling.

Bakun, MagdaInstitute of Biochemistry and Biophysics of the Polish Academy of Sciences (författare)
Świderska, BiankaInstitute of Biochemistry and Biophysics of the Polish Academy of Sciences (författare)
Dudkiewicz, MałgorzataWarsaw University of Life Sciences (författare)
Koscielny, AlicjaInternational Institute of Molecular and Cell Biology, Warsaw (författare)
Bajur, AnnaWarsaw University of Life Sciences,International Institute of Molecular and Cell Biology, Warsaw,Max Planck Institute of Molecular Cell Biology and Genetics (författare)
Jaworski, JacekInternational Institute of Molecular and Cell Biology, Warsaw (författare)
Dadlez, MichałInstitute of Biochemistry and Biophysics of the Polish Academy of Sciences (författare)
Pawłowski, KrzysztofLund University,Lunds universitet,Institutionen för translationell medicin,Medicinska fakulteten,Department of Translational Medicine,Faculty of Medicine,Warsaw University of Life Sciences(Swepub:lu)med-kzp (författare)
International Institute of Molecular and Cell Biology, WarsawWarsaw University of Life Sciences (creator_code:org_t)

Av författaren/redakt...: Hareza, Agnieszk ...; Bakun, Magda; Świderska, Biank ...; Dudkiewicz, Małg ...; Koscielny, Alicj ...; Bajur, Anna; visa fler...; Jaworski, Jacek; Dadlez, Michał; Pawłowski, Krzys ...; visa färre...

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