LIM-only protein Lmo2 forms a protein complex with erythroid transcription factor GATA-1

• The LIM-only protein Lmo2, originally identified as an oncogenic protein in human T cell leukemia, is essential for erythropoiesis. A possible role for Lmo2 in transcription during erythropoiesis has been investigated. Direct interaction of Lmo2 was observed in vitro and in vivo with the zinc finger transcription factor GATA-1, as well as with the basic helix-loop-helix (bHLH) transcription factor Tall. By using mammalian two-hybrid analysis, E47/Tall/Lmo2/GATA-1 protein complex could be demonstrated. Thus, a molecular link exists between three proteins crucial for erythropoiesis. This data suggest that variations in amounts of complexes involving Lmo2, Tall, and GATA-1 could be important for erythroid differentiation.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Hematologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Hematology (hsv//eng)

Nyckelord

Adaptor Proteins, Signal Transducing

Animals

Basic Helix-Loop-Helix Transcription Factors

COS Cells

DNA-Binding Proteins

Erythroid-Specific DNA-Binding Factors

Erythropoiesis

GATA1 Transcription Factor

Helix-Loop-Helix Motifs

Humans

LIM Domain Proteins

Leukemia, Erythroblastic, Acute

Leukemia, T-Cell

Metalloproteins

Proto-Oncogene Proteins

Recombinant Proteins

Transcription Factors

Transcription, Genetic

Transfection

Tumor Cells, Cultured

Zinc Fingers

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)