KATANIN and CLASP function at different spatial scales to mediate microtubule response to mechanical stress in Arabidopsis cotyledons

Mechanical stress influences cell- and tissue-scale processes across all kingdoms. It remains challenging to delineate how mechanical stress, originating at these different length scales, impacts cell and tissue form. We combine growth tracking of cells, quantitative image analysis, as well as molecular and mechanical perturbations to address this problem in pavement cells of Arabidopsis thaliana cotyledon tissue. We show that microtubule organization based on chemical signals and cell-shape-derived mechanical stress varies during early stages of pavement cell development and is mediated by the evolutionary conserved proteins, KATANIN and CLASP. However, we find that these proteins regulate microtubule organization in response to tissue-scale mechanical stress to different extents in the cotyledon epidermis. Our results further demonstrate that regulation of cotyledon form is uncoupled from the mechanical-stress-dependent control of pavement cell shape that relies on microtubule organization governed by subcellular mechanical stress.

Schneider, RenéMax-Planck-Institute for Molecular Plant Physiology (författare)
Matz, Timon W.University of Potsdam,Max-Planck-Institute for Molecular Plant Physiology (författare)
Carter, RossUniversity of Cambridge (författare)
Ehrhardt, David W.Stanford University,Carnegie Institution for Science (författare)
Jönsson, HenrikLund University,Lunds universitet,Beräkningsbiologi och biologisk fysik - Har omorganiserats,Institutionen för astronomi och teoretisk fysik - Har omorganiserats,Naturvetenskapliga fakulteten,Computational Biology and Biological Physics - Has been reorganised,Department of Astronomy and Theoretical Physics - Has been reorganised,Faculty of Science,University of Cambridge(Swepub:lu)he5413jo (författare)
Nikoloski, ZoranMax-Planck-Institute for Molecular Plant Physiology,University of Potsdam (författare)
Sampathkumar, ArunMax-Planck-Institute for Molecular Plant Physiology (författare)
Max-Planck-Institute for Molecular Plant PhysiologyUniversity of Potsdam (creator_code:org_t)

Av författaren/redakt...: Eng, Ryan C.; Schneider, René; Matz, Timon W.; Carter, Ross; Ehrhardt, David ...; Jönsson, Henrik; visa fler...; Nikoloski, Zoran; Sampathkumar, Ar ...; visa färre...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.