Intra- and extracellular regulation of activity and processing of legumain by cystatin E/M

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Smith, Robert (author)

Intra- and extracellular regulation of activity and processing of legumain by cystatin E/M

Article/chapterEnglish2012

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Elsevier BV,2012

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LIBRIS-ID:oai:lup.lub.lu.se:271ed8d4-890a-484e-ad07-d2031fec8895
https://lup.lub.lu.se/record/3512186URI
https://doi.org/10.1016/j.biochi.2012.07.026DOI

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Language:English
Summary in:English

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Subject category:ref swepub-contenttype

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Legumain, an asparaginyl endopeptidase, is up-regulated in tumour and tumour-associated cells, and is linked to the processing of cathepsin B, L, and proMMP-2. Although legumain is mainly localized to the endosomal/lysosomal compartments, legumain has been reported to be localized extracellularly in the tumour microenvironrnent and associated with extracellular matrix and cell surfaces. The most potent endogenous inhibitor of legumain is cystatin E/M, which is a secreted protein synthesised with an export signal. Therefore, we investigated the cellular interplay between legumain and cystatin E/M. As a cell model. HEK293 cells were transfected with legumain cDNA, cystatin E/M cDNA, or both, and over-expressing monoclonal cell lines were selected (termed M38L, M4C, and M3CL, respectively). Secretion of prolegumain from M38L cells was inhibited by treatment with brefeldin A, whereas bafilomycin A1 enhanced the secretion. Cellular processing of prolegumain to the 46 and 36 kDa enzymatically active forms was reduced by treatment with either substance alone. M38L cells showed increased, but M4C cells decreased, cathepsin L processing suggesting a crucial involvement of legumain activity. Furthermore, we observed internalization of cystatin E/M and subsequently decreased intracellular legumain activity. Also, prolegumain was shown to internalize followed by increased intracellular legumain processing and activation. In addition, in M4C cells incomplete processing of the internalized prolegumain was observed, as well as nuclear localized cystatin E/M. Furthermore, auto-activation of secreted prolegumain was inhibited by cystatin E/M, which for the first time shows a regulatory role of cystatin E/M in controlling both intra- and extracellular legumain activity. (C) 2012 Elsevier Masson SAS. All rights reserved.

Subject headings and genre

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
Asparaginyl endopeptidase
AEP
Legumain
Cystatin E/M
Cathepsin B
Cathepsin L

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Johansen, Harald T. (author)
Nilsen, Hilde (author)
Haugen, Mads H. (author)
Pettersen, Solveig J. (author)
Maelandsmo, Gunhild M. (author)
Abrahamson, MagnusLund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)kkem-mab (author)
Solberg, Rigmor (author)
Avdelningen för klinisk kemi och farmakologiInstitutionen för laboratoriemedicin (creator_code:org_t)

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In:Biochimie: Elsevier BV94:12, s. 2590-25991638-61830300-9084

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NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

Articles in the publication: Biochimie

By the university: Lund University

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