Immunofluorescent demonstration of the presence of protein HC on the surface of human lymphocytes

• Indirect immunofluorescence was used to demonstrate the presence of protein HC on the surface of a large percentage of normal human peripheral blood lymphocytes. Protein HC is a recently described charge heterogeneous, complex-forming glycoprotein normally present in human plasma, urine and cerebrospinal fluid. The synthesis of protein HC by lymphocytes was indicated by the removal of the glycoprotein from the cell surfaces on trypsinization of the cells followed by the reappearance of the protein on continued cultivation of the cells.

Keyword

Cell Membrane/metabolism

Female

Fluorescent Antibody Technique

Glycoproteins/blood

Humans

Lymphocytes/metabolism

Male

Publication and Content Type

art (subject category)

ref (subject category)