Processing and secretion of rat alpha 1-microglobulin-bikunin expressed in eukaryotic cell lines

• The precursor protein alpha 1-microglobulin-bikunin was cleaved to the same degree whether expressed in CHO cells or in mutated CHO cells, RPE.40 cells, suggested to lack a functional form of the intracellular protease furin. Thus, alpha 1-microglobulin-bikunin probably is not cleaved in vivo by furin. However, simultaneous overexpression of the precursor and furin in COS, CHO and RPE.40 cells increased the cleavage, suggesting that compartmentalisation and concentrations of protease and precursor are important for the cleavage, besides the in vitro specificity. Expression of alpha 1-microglobulin and bikunin alone gave different protein patterns of SDS-PAGE as compared to expression of the precursor and subsequent cleavage, suggesting that the precursor protein is important for the post-translational handling of alpha 1-microglobulin and bikunin.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Immunology in the medical area (hsv//eng)

Nyckelord

Alpha-Globulins/chemistry

Animals

Base Sequence

CHO Cells

Cricetinae

Furin

Gene Expression

Glycoproteins/chemistry

Membrane Glycoproteins

Molecular Sequence Data

Molecular Weight

Protease Inhibitors/metabolism

Protein Precursors/genetics

Protein Processing, Post-Translational/physiology

Rats

Subtilisins/genetics

Transfection

Trypsin Inhibitor, Kunitz Soybean

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)