L773:0014 5793 OR L773:1873 3468
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- Bratt, TLund University (author)
Processing and secretion of rat alpha 1-microglobulin-bikunin expressed in eukaryotic cell lines
- Article/chapterEnglish1994
Publisher, publication year, extent ...
- 2001-10-18
- Wiley,1994
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:2a9d0aa5-1407-4a2b-8b09-c40bdbca97f8
- https://lup.lub.lu.se/record/2a9d0aa5-1407-4a2b-8b09-c40bdbca97f8URI
- https://doi.org/10.1016/0014-5793(94)01087-0DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- The precursor protein alpha 1-microglobulin-bikunin was cleaved to the same degree whether expressed in CHO cells or in mutated CHO cells, RPE.40 cells, suggested to lack a functional form of the intracellular protease furin. Thus, alpha 1-microglobulin-bikunin probably is not cleaved in vivo by furin. However, simultaneous overexpression of the precursor and furin in COS, CHO and RPE.40 cells increased the cleavage, suggesting that compartmentalisation and concentrations of protease and precursor are important for the cleavage, besides the in vitro specificity. Expression of alpha 1-microglobulin and bikunin alone gave different protein patterns of SDS-PAGE as compared to expression of the precursor and subsequent cleavage, suggesting that the precursor protein is important for the post-translational handling of alpha 1-microglobulin and bikunin.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Immunologi inom det medicinska området hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Immunology in the medical area hsv//eng
- Alpha-Globulins/chemistry
- Animals
- Base Sequence
- CHO Cells
- Cricetinae
- Furin
- Gene Expression
- Glycoproteins/chemistry
- Membrane Glycoproteins
- Molecular Sequence Data
- Molecular Weight
- Protease Inhibitors/metabolism
- Protein Precursors/genetics
- Protein Processing, Post-Translational/physiology
- Rats
- Subtilisins/genetics
- Transfection
- Trypsin Inhibitor, Kunitz Soybean
Added entries (persons, corporate bodies, meetings, titles ...)
- Cedervall, TLund University,Lunds universitet,Medicinska fakulteten,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Faculty of Medicine,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)bpc-cto (author)
- Akerström, BLund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)medk-bak (author)
- Lund UniversityMedicinska fakulteten (creator_code:org_t)
Related titles
- In:FEBS Letters: Wiley354:1, s. 57-610014-5793
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