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Radar plots facilit...
Radar plots facilitate differential diagnosis of acute promyelocytic leukemia and NPM1+ acute myeloid leukemia by flow cytometry
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- Gupta, Monali (författare)
- King's College Hospital,University Health Network University of Toronto
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- Jafari, Katayoon (författare)
- University of Saskatchewan,University Health Network University of Toronto
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- Rajab, Amr (författare)
- LifeLabs,University Health Network University of Toronto
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- Wei, Cuihong (författare)
- University of Saskatchewan,University Health Network University of Toronto
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- Mazur, Joanna (författare)
- University of Zielona Góra,National Research Institute of Mother and Child
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- Tierens, Anne (författare)
- University Health Network University of Toronto
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- Hyjek, Elizabeth (författare)
- University of Chicago,University Health Network University of Toronto
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- Musani, Rumina (författare)
- University Health Network University of Toronto
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- Porwit, Anna (författare)
- Lund University,Lunds universitet,Patologi, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Pathology, Lund,Section V,Department of Clinical Sciences, Lund,Faculty of Medicine,University Health Network University of Toronto
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(creator_code:org_t)
- 2020-12-10
- 2021
- Engelska.
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Ingår i: Cytometry Part B - Clinical Cytometry. - : Wiley. - 1552-4949 .- 1552-4957. ; 100:4, s. 409-420
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://onlinelibrar...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background: Acute promyelocytic leukemia (APL) is one of the most life-threatening hematological emergencies and requires a prompt correct diagnosis by cytomorphology and flow cytometry (FCM) with later confirmation by cytogenetics/molecular genetics. However, nucleophosmin 1 muted acute myeloid leukemia (NPM1+ AML) can mimic APL, especially the hypogranular variant of APL. Our study aimed to develop a novel, Radar plot-based FCM strategy to distinguish APLs and NPM1+ AMLs quickly and accurately. Method: Diagnostic samples from 52 APL and 32 NPM1+ AMLs patients were analyzed by a 3-tube panel of 10-color FCM. Radar plots combining all markers were constructed for each tube. Percentages of positive leukemic cells and mean fluorescence intensity were calculated for all the markers. Results: APL showed significantly higher expression of CD64, CD2, and CD13, whereas more leukemic cells were positive for CD11b, CD11c, CD15, CD36, and HLA-DR in NPM1+ AMLs. Radar plots featured CD2 expression, a lack of a monocytic component, lack of expression of HLA-DR and CD15, and a lack of a prominent CD11c+ population as recurring characteristics of APL. The presence of blasts with low SSC, presence of at least some monocytes, some expression of HLA-DR and/or CD15, and a prominent CD11c population were recurrent characteristics of NPM1+ AMLs. Radar plot analysis could confidently separate all hypergranular APL cases from any NPM1+ AML and in 90% of cases between variant APL and blastic NPM1+ AML. Conclusion: Radar plots can potentially add to differential diagnostics as they exhibit characteristic patterns distinguishing APL and different types of NPM1+ AMLs.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
Nyckelord
- acute promyelocytic leukemia
- flow cytometry
- mean fluorescence intensity
- NPM1 mutated acute myeloid leukemia
- radar plot
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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