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Identification of T...
Identification of TPM2 and CNN1 as Novel Prognostic Markers in Functionally Characterized Human Colon Cancer-Associated Stromal Cells
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- Mele, Valentina (author)
- University Hospital Basel
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- Basso, Camilla (author)
- Regional Hospital of Lugano,Università della Svizzera Italiana
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- Governa, Valeria (author)
- Lund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumörmikromiljön,Forskargrupper vid Lunds universitet,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University Research Groups
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- Glaus Garzon, Jesus F. (author)
- University of Zurich
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- Muraro, Manuele G. (author)
- University Hospital Basel
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- Däster, Silvio (author)
- University Hospital Basel
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- Nebiker, Christian A. (author)
- University Hospital Basel
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- Mechera, Robert (author)
- University Hospital Basel
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- Bolli, Martin (author)
- University Hospital Basel
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- Schmidt, Alexander (author)
- University of Basel
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- Geiger, Roger (author)
- Università della Svizzera Italiana
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Spagnoli, Giulio C. (author)
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- Christoforidis, Dimitri (author)
- Università della Svizzera Italiana,Regional Hospital of Lugano
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- Majno, Pietro E. (author)
- Regional Hospital of Lugano,Università della Svizzera Italiana
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- Borsig, Lubor (author)
- University of Zurich
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- Iezzi, Giandomenica (author)
- Università della Svizzera Italiana,Regional Hospital of Lugano
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(creator_code:org_t)
- 2022-04-16
- 2022
- English.
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In: Cancers. - : MDPI AG. - 2072-6694. ; 14:8
- Related links:
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http://dx.doi.org/10... (free)
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Abstract
Subject headings
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- Stromal infiltration is associated with poor prognosis in human colon cancers. However, the high heterogeneity of human tumor-associated stromal cells (TASCs) hampers a clear identification of specific markers of prognostic relevance. To address these issues, we established short-term cultures of TASCs and matched healthy mucosa-associated stromal cells (MASCs) from human primary colon cancers and, upon characterization of their phenotypic and functional profiles in vitro and in vivo, we identified differentially expressed markers by proteomic analysis and evaluated their prognostic significance. TASCs were characterized by higher proliferation and differentiation potential, and enhanced expression of mesenchymal stem cell markers, as compared to MASCs. TASC triggered epithelial–mesenchymal transition (EMT) in tumor cells in vitro and promoted their metastatic spread in vivo, as assessed in an orthotopic mouse model. Proteomic analysis of matched TASCs and MASCs identified a panel of markers preferentially expressed in TASCs. The expression of genes encoding two of them, calponin 1 (CNN1) and tropomyosin beta chain isoform 2 (TPM2), was significantly associated with poor outcome in independent databases and outperformed the prognostic significance of currently proposed TASC markers. The newly identified markers may improve prognostication of primary colon cancers and identification of patients at risk.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- CNN1
- colon cancer
- prognostic markers
- proteomics
- TPM2
- tumor-associated stromal cells
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Mele, Valentina
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Basso, Camilla
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Governa, Valeria
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Glaus Garzon, Je ...
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Muraro, Manuele ...
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Däster, Silvio
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Nebiker, Christi ...
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Mechera, Robert
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Bolli, Martin
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Schmidt, Alexand ...
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Geiger, Roger
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Spagnoli, Giulio ...
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Christoforidis, ...
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Majno, Pietro E.
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Borsig, Lubor
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Iezzi, Giandomen ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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Cancers
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Lund University