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Recombining germlin...
Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries
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- Söderlind, Eskil (author)
- BioInvent Therapeutic AB
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- Strandberg, Leif (author)
- BioInvent Therapeutic AB
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- Jirholt, Pernilla (author)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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- Kobayashi, Norihiro (author)
- Tohoku University
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- Alexeiva, Vessela (author)
- BioInvent Therapeutic AB
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- Åberg, Anna Maria (author)
- BioInvent Therapeutic AB
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- Nilsson, Anna (author)
- BioInvent Therapeutic AB
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- Jansson, Bo (author)
- BioInvent Therapeutic AB
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- Ohlin, Mats (author)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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- Wingren, Christer (author)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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- Danielsson, Lena (author)
- BioInvent Therapeutic AB
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- Carlsson, Roland (author)
- BioInvent Therapeutic AB
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- Borrebaeck, Carl A K (author)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- Springer Science and Business Media LLC, 2000
- 2000
- English 5 s.
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In: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1087-0156 .- 1546-1696. ; 18:8, s. 852-856
- Related links:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
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- We constructed a single-chain Fv antibody library that permits human complementarity-determining region (CDR) gene fragments of any germline to be incorporated combinatorially into the appropriate positions of the variable-region frameworks VH-DP47 and VL-DPL3. A library of 2 x 109 independent transformants was screened against haptens, peptides, carbohydrates, and proteins, and the selected antibody fragments exhibited dissociation constants in the subnanomolar range. The antibody genes in this library were built on a single master framework into which diverse CDRs were allowed to recombine. These CDRs were sampled from in vivo-processed gene sequences, thus potentially optimizing the levels of correctly folded and functional molecules, and resulting in a molecule exhibiting a lower computed immunogenicity compared to naive immunoglobulins. Using the modularized assembly process to incorporate foreign sequences into an immunoglobulin scaffold, it is possible to vary as many as six CDRs at the same time, creating genetic and funcfional variation in antibody molecules.
Keyword
- Antibody engineering
- Diversity
- In vitro evolution
- Recombination
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Söderlind, Eskil
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Strandberg, Leif
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Jirholt, Pernill ...
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Kobayashi, Norih ...
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Alexeiva, Vessel ...
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Åberg, Anna Mari ...
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show more...
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Nilsson, Anna
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Jansson, Bo
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Ohlin, Mats
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Wingren, Christe ...
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Danielsson, Lena
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Carlsson, Roland
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Borrebaeck, Carl ...
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- Articles in the publication
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Nature Biotechno ...
- By the university
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Lund University