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  • Nilsson, RuneLund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine (author)

Different protein A immunosorbents may have different binding specificity for rat immunoglobulins

  • Article/chapterEnglish1983

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  • 1983

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  • LIBRIS-ID:oai:lup.lub.lu.se:2ff95b87-5036-49b9-90b7-d6e8f383acef
  • https://lup.lub.lu.se/record/1103043URI
  • https://doi.org/10.1016/0022-1759(83)90251-XDOI

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  • Language:English
  • Summary in:English

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  • Purified polyclonal immunoglobulin preparations representing the 4 rat IgG subclasses were tested for binding to Staphylococcus aureus protein A attached to 3 different solid phases (Staphylococcus aureus Cowan I bacteria, Sepharose CL4B and Sepharose 6MB). Protein A Sepharose CL4B showed higher reactivity with IgG1 and IgG2b than the staphylococci, whereas protein A Sepharose 6MB showed a lower uptake of these subclasses. No differences were seen for IgG2a and IgG2c. Protein A on different solid phases cannot be used interchangeably without confirmation of binding specificity.

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  • Myhre, E (author)
  • Kronvall, G (author)
  • Sjögren, H O (author)
  • Bröstcancer-genetikSektion I (creator_code:org_t)

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  • In:Journal of Immunological Methods62:2, s. 241-2451872-7905

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Nilsson, Rune
Myhre, E
Kronvall, G
Sjögren, H O
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MEDICAL AND HEALTH SCIENCES
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