Tetratricopeptide-containing SMALL KERNEL 11 is essential for the assembly of cytochrome c oxidase in maize mitochondria

Assembly of the functional complexes of the mitochondrial respiratory chain requires sophisticated and efficient regulatory mechanisms. In plants, the subunit composition and assembly factors involved in the biogenesis of cytochrome c oxidase (complex IV) are substantially less defined than in mammals and yeast. In this study, we cloned maize (Zea mays) Small kernel 11 (Smk11) via map-based cloning. Smk11 encodes a mitochondria-localized tetratricopeptide repeat protein. Disruption of Smk11 severely affected the assembly and activity of mitochondrial complex IV, leading to delayed plant growth and seed development. Protein interactions studies revealed that SMK11 might interact with four putative complex IV assembly factors, Inner membrane peptidase 1A (ZmIMP1A), MYB domain protein 3R3 (ZmMYB3R-3), cytochrome c oxidase 23 (ZmCOX23), and mitochondrial ferredoxin 1 (ZmMFDX1), among which ZmMFDX1 might interact with subunits ZmCOX6a and ZmCOX-X1; ZmMYB3R-3 might also interact with ZmCOX6a. The mutation of SMK11 perturbed the normal assembly of these subunits, leading to the inactivation of complex IV. The results of this study revealed that SMK11 serves as an accessory assembly factor required for the normal assembly of subunits into complex IV, which will accelerate the elucidation of the assembly of complex IV in plant mitochondria.

Fan, KaijianChinese Academy of Agricultural Sciences (författare)
Zhen, SihanChina Agricultural University (författare)
Zhang, JieChina Agricultural University (författare)
Liu, YanChinese Academy of Agricultural Sciences (författare)
Fu, JunjieChinese Academy of Agricultural Sciences (författare)
Qi, ChunlaiChinese Academy of Agricultural Sciences (författare)
Wei, QianhanChina Agricultural University (författare)
Du, YaoChina Agricultural University (författare)
Tatar, WurinileChinese Academy of Agricultural Sciences (författare)
Zhang, XiaofengChinese Academy of Agricultural Sciences (författare)
Wang, GuoyingChinese Academy of Agricultural Sciences (författare)
Rasmusson, Allan G.Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Växtbiologi,Forskargrupper vid Lunds universitet,Molecular Cell Biology,Department of Biology,Faculty of Science,Plant Biology,Lund University Research Groups(Swepub:lu)fysb-ara (författare)
Wang, JianhuaChina Agricultural University (författare)
Liu, YunjunChinese Academy of Agricultural Sciences (författare)
Chinese Academy of Agricultural SciencesChina Agricultural University (creator_code:org_t)

Ingår i:Plant Physiology: Oxford University Press (OUP)192:1, s. 170-1870032-08891532-2548

Av författaren/redakt...: Ren, Zhenjing; Fan, Kaijian; Zhen, Sihan; Zhang, Jie; Liu, Yan; Fu, Junjie; visa fler...; Qi, Chunlai; Wei, Qianhan; Du, Yao; Tatar, Wurinile; Zhang, Xiaofeng; Wang, Guoying; Rasmusson, Allan ...; Wang, Jianhua; Liu, Yunjun; visa färre...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.