Protein-surfactant interactions at solid surfaces

• Effects of surfactants on protein adsorption are reviewed. Differences between removal of preadsorbed proteins (elutability) and competitive adsorption are discussed and simple models are suggested. It can be concluded that surfactants may interact through solubilization or replacement mechanisms depending on surfactant- surface interactions and surfactant- protein binding. Solubilization requires complex formation between protein and surfactant, and the replacement adsorption of the surfactant to the surface. As for protein adsorption, one of the most important properties affecting the elutability appears to be the conformational stability. Differences between a competitive situation and addition of surfactant after adsorption of the protein are suggested to originate from alteration in surface activity of protein-surfactant complexes formed in solution as compared to pure protein, the difference in diffusivity of surfactants and protein, and time dependent conformational changes of the protein.

Ämnesord

NATURVETENSKAP  -- Kemi -- Fysikalisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Physical Chemistry (hsv//eng)

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