Sökning: WFRF:(Moller T.)
> (1990-1994) >
Isolation of the ro...
-
Rasmusson, A. G.Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science
(författare)
Isolation of the rotenome-sensitive NADH-ubiquinone reductase (complex I) from red beet mitochondria
- Artikel/kapitelEngelska1994
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
-
LIBRIS-ID:oai:lup.lub.lu.se:3407dfee-7c7d-4c09-8159-4dcc30069206
-
https://lup.lub.lu.se/record/3407dfee-7c7d-4c09-8159-4dcc30069206URI
-
https://doi.org/10.1034/j.1399-3054.1994.900324.xDOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:art swepub-publicationtype
-
Ämneskategori:ref swepub-contenttype
Anmärkningar
-
Complex 1 of the respirator) chain (EC 1.6.531, measured as NADH-duroquinone and NADH-ubiquinone, reductase activities, was isolated from purified red beetroot (Beta vulgaris L.I mitochondria. The mitochondria were disrupted by freeze-thawing and inner membrane vesicles were pelleted. After solubilization of the vesicles with Triton X-100, the enzyme complex was purified 11-fold (compared to the activity in the inner membrane vesicles) by size-exclusion chromatography on a Sephacryl S-400 HR column and then by ion-exchange chromatography on a DEAE-Sepharose CL-6B column. Triton X-100 was present throughout the purification procedure. Tire purified complex showed approximately 30 bands on SDS-PAGE and about 15 polypeptides including those at 80. 54, 53. 51. 27. 25 and 22 kDa cross-reacted with polyclonal antibodies raised against complex I from Neurospora crassa. This is similar lo the pattern obtained with complex I from Neurospera crassa.Analysis by nativc-SDS 2-dimensional PAGE revealed the existence of several molecular mass forms of the purified complex.After reconstitution of the purified complex into phosphatidylcholine vesicles, the NADH-ubiquinone reductase activity had a Km (NADH) of about I μM and was inhibited by both rotenone and dicyclohexylcarbodiimide.
Ämnesord och genrebeteckningar
-
Beta vulgaris
-
complex I
-
inner membrane
-
mitochondrion
-
NADH dehydrogenase
-
red beetroot
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Mendel-Hartvig, J.University of Oregon
(författare)
-
Moller, Ian M.
(författare)
-
Wiskich, J. T.University of Adelaide
(författare)
-
Molekylär cellbiologiBiologiska institutionen
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Physiologia Plantarum: Wiley90:3, s. 607-6150031-93171399-3054
Internetlänk
Hitta via bibliotek
Till lärosätets databas