Cloning and functional expression of the guinea pig neuropeptide Y Y2 receptor

• Five neuropeptide Y (NPY) receptor subtypes have been cloned in mammals. The degree of sequence conservation differs considerably between subtypes as well as between evolutionary lineages. To shed further light on this, we have cloned the five NPY receptors in the guinea pig. Here, we report the cloning of the guinea pig Y2 receptor. The Y2 receptor is generally highly conserved, with 90-95% identity between different orders of mammals, including the guinea pig. The guinea pig receptor has a divergent cytoplasmic tail, indicating possible differences in regulation of signalling and/or down regulation. COS-7 cells transiently transfected with the gpY2 receptor show saturable 125I-PYY binding with a Kd = 6 pM. In displacement experiments, the gpY2 receptor was similar to the human and rat receptors with the following rank order of potencies: pNPY > pPYY > pNPY13-36 = pNPY22-36 > [Leu31Pro34]NPY > BIBP3226. Thus, the guinea pig Y2 receptor is well conserved in comparison with human and rat with regard to both amino acid sequence and pharmacological profile.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)

Nyckelord

Amino Acid Sequence

Animals

Arginine/analogs & derivatives

COS Cells

Cloning, Molecular

Conserved Sequence

Gene Expression

Guinea Pigs

Humans

In Vitro Techniques

Kinetics

Molecular Sequence Data

Neuropeptide Y/analogs & derivatives

Peptide Fragments/metabolism

Peptide YY/metabolism

Rats

Receptors, Neuropeptide Y/chemistry

Recombinant Proteins/chemistry

Sequence Homology, Amino Acid

Transfection

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)