Effect of cholesterol on the lateral nanoscale dynamics of fluid membranes

Inelastic neutron scattering was used to study the effect of 5 and 40 mol% cholesterol on the lateral nanoscale dynamics of phospholipid membranes. By measuring the excitation spectrum at several lateral q (||) values (up to q (||) = 3 (-1)), complete dispersion curves were determined of gel, fluid and liquid-ordered phase bilayers. The inclusion of cholesterol had a distinct effect on the collective dynamics of the bilayer's hydrocarbon chains; specifically, we observed a pronounced stiffening of the membranes on the nanometer length scale in both gel and fluid bilayers, even though they were experiencing a higher degree of molecular disorder. Also, for the first time we determined the nanoscale dynamics in the high-cholesterol liquid-ordered phase of bilayers containing cholesterol. Namely, this phase appears to be "softer" than fluid bilayers, but better ordered than bilayers in the gel phase.

Barrett, Matthew A. (författare)
Hiess, ArnoLund University,Lunds universitet,European Spallation Source ESS AB,Stiftelser och övriga anknutna verksamheter,Other Institutions and Utilities(Swepub:lu)esss-ahs (författare)
Salditt, Tim (författare)
Katsaras, John (författare)
Shi, An-Chang (författare)
Rheinstaedter, Maikel C. (författare)
European Spallation Source ESS ABStiftelser och övriga anknutna verksamheter (creator_code:org_t)

Ingår i:European Biophysics Journal: Springer Science and Business Media LLC41:10, s. 901-9130175-75711432-1017

Av författaren/redakt...: Armstrong, Clare ...; Barrett, Matthew ...; Hiess, Arno; Salditt, Tim; Katsaras, John; Shi, An-Chang; visa fler...; Rheinstaedter, M ...; visa färre...

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