ACouWash : A standalone instrument for the washing, separation and enrichment of cells.

• We developed the AcouWash (Figures 1 and 2), a benchtop instrument for flow-through cell and particle separation by acoustophoresis. Building on previous research [1-4] AcouWash can re-suspend cells in new medium, separate cells based on size or acoustic properties, or enrich cells, Figure 3. We present our initial evaluation of AcouWash regarding size-based separation of particles, cell washing, cell viability, and time savings compared to centrifugation. We repeatedly recovered 90-100% of target cells and particles, washed away 99.9% of a small fluorescent tracer molecule, viability of cells was unchanged, and reduced the time for standard cell labelling by 50%.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Other Basic Medicine (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Annan medicinsk bioteknologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Other Medical Biotechnology (hsv//eng)

Nyckelord

Acoustofluidics

Acoustophoresis

Blood cells

Enrichment

Separation

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