id:"swepub:oai:lup.lub.lu.se:3e046f81-564e-44ef-a7ef-f7e326b05d00"
Sökning: id:"swepub:oai:lup.lub.lu.se:3e046f81-564e-44ef-a7ef-f7e326b05d00" > Gene expression pro...
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- Seth, A (författare)
Gene expression profiling of ductal carcinomas in situ and invasive breast tumors
- Artikel/kapitelEngelska2003
Förlag, utgivningsår, omfång ...
- 2003
Nummerbeteckningar
- LIBRIS-ID:oai:lup.lub.lu.se:3e046f81-564e-44ef-a7ef-f7e326b05d00
- https://lup.lub.lu.se/record/305800URI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
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- SwePubSwePub
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Anmärkningar
- Comparative and functional genomics are powerful tools to advance the understanding of the molecular basis of cancer. It is believed that genes are epigenetically regulated and, thus, each tumor type and stage will be characterized by a gene expression fingerprint. In this study we identified genes that are differentially expressed in ductal carcinoma in situ and invasive ductal carcinoma of the breast. To isolate genes that are associated with progression of breast cancer we performed differential display and subtractive cloning procedures using matched RNA from normal and tumor tissue. cDNA microarray analysis generated gene expression profiles typical of the transition front in situ to invasive breast cancer when we used mRAA extracted from a case of low-to intermediate-grade DCIS and a case of high-grade DC1S/IDC. cDNAs from these samples were the probes in a cDNA microarray hybridization to 9183 unique cDAAs representing 8507 genes. Signals from both transcriptomes were obtained for 8083 genes, and the balanced differential expression values between pure DCIS and DCIS/invasive tumors revealed 303 distinct cDNAs with a ratio of > 2. Interferon inducible genes were found to be expressed at the highest level in the pure DCIS sample. Genes most abundantly expressed in the invasive tumor were immunoglobulin heavy constant gamma 3 and calgranulin B. Further analysis of RNA and protein expression in breast tumor cell lines and patient tissue samples revealed that: IGFBP-rP1 is down-regulated in invasive tumors whereas cyclin I protein is regulated by ubiquitination and is associated with ER-negative breast cancers. Conclusion: The known and novel genes discussed here represent targets for molecular characterization during breast cancer development as well as,for designing novel strategies for diagnosis and treatment.
Ämnesord och genrebeteckningar
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Cancer och onkologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Cancer and Oncology hsv//eng
- interferon genes
- genomics
- breast cancer
- gene expression profiling
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Kitching, R (författare)
- Landberg, GöranLund University,Lunds universitet,Patologi, Malmö,Forskargrupper vid Lunds universitet,Pathology, Malmö,Lund University Research Groups(Swepub:lu)pat-gla (författare)
- Xu, J (författare)
- Zubovits, J (författare)
- Burger, AM (författare)
- Patologi, MalmöForskargrupper vid Lunds universitet (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Anticancer research23:3A, s. 2043-20511791-7530
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- Seth, A
- Kitching, R
- Landberg, Göran
- Xu, J
- Zubovits, J
- Burger, AM
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
- MEDICIN OCH HÄLS ...
- och Klinisk medicin
- och Cancer och onkol ...
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- Anticancer resea ...
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- Lunds universitet
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