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  • Horvatovich, Péter (author)

In vitro Transcription/Translation System: A Versatile Tool in the Search for Missing Proteins

  • Article/chapterEnglish2015

Publisher, publication year, extent ...

  • 2015-07-28
  • American Chemical Society (ACS),2015

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:3fbea94d-315f-4f5a-a8a4-3153d44f7f90
  • https://lup.lub.lu.se/record/7520337URI
  • https://doi.org/10.1021/acs.jproteome.5b00486DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Approximately 18% of all human genes purported to encode proteins have not been directly evidenced at the protein level, according to the validation criteria established by neXtProt, and are considered as “missing” proteins. One of the goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to identify as many of these “missing” proteins as possible in human samples using mass spectrometry-based methods. To further this goal, a consortium of C–HPP teams (chromosomes 5, 10, 16 and 19) has joined forces to devise new strategies to identify “missing” proteins by use of a cell-free in vitro transcription/translation system (IVTT). The proposed strategy employs LC-MS/MS data-dependent acquisition (DDA) and targeted selective reaction monitoring (SRM) methods to scrutinize low complexity samples derived from IVTT translation. The optimized assays are then applied to identify “missing” proteins in human cells and tissues. We describe the approach and show proof-of-concept results for development of LC-SRM assays for identification of eighteen “missing” proteins. We believe that the IVTT system, when coupled with downstream mass spectrometric identification, can be applied to identify proteins that have eluded more traditional methods of detection.

Subject headings and genre

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  • Végvári, ÁkosLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)elma-asv (author)
  • Saul, Justin (author)
  • Park, Jin (author)
  • Qiu, Ji (author)
  • Syring, Michael (author)
  • Pirrotte, Patrick (author)
  • Petritis, Konstantinos (author)
  • Tegeler, Tony (author)
  • Aziz, Meraj (author)
  • Fuentes, Manuel (author)
  • Diez, Paula (author)
  • Gonzalez-Gonzalez, Maria (author)
  • Ibarrola, Nieves (author)
  • Droste, Conrad (author)
  • De Las Rivas, Javier (author)
  • Gil, Concha (author)
  • Clemente, Felipe (author)
  • Hernaez, Maria Luisa (author)
  • Corrales, Fernando (author)
  • Nilsson, Carol (author)
  • Berven, Frode (author)
  • Bischoff, Rainer (author)
  • Fehniger, ThomasLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)elma-tfr (author)
  • LaBaer, Joshua (author)
  • Marko-Varga, GyörgyLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)akem-gmv (author)
  • Avdelningen för Biomedicinsk teknikInstitutionen för biomedicinsk teknik (creator_code:org_t)

Related titles

  • In:Journal of Proteome Research: American Chemical Society (ACS)14:9, s. 3441-34511535-38931535-3907

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