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Release of dog poly...
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Björk, PeterLund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Department of Clinical Sciences, Malmö,Faculty of Medicine,Skåne University Hospital
(författare)
Release of dog polymorphonuclear leukocyte cathepsin g, normally and in endotoxin and pancreatitic shock : Isolation and partial characterization of dog polymorphonuclear leukocyte cathepsin g
- Artikel/kapitelEngelska1991
Förlag, utgivningsår, omfång ...
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Walter de Gruyter GmbH,1991
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8 s.
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LIBRIS-ID:oai:lup.lub.lu.se:40739ca1-630a-498f-94cf-ff652bbfa039
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https://lup.lub.lu.se/record/40739ca1-630a-498f-94cf-ff652bbfa039URI
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https://doi.org/10.1515/bchm3.1991.372.1.419DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
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Dog polymorphonuclear leukocyte cathepsin G was isolated from a granule extract using a two-step procedure including affinity chromatography on aTrasylol-Sepharose gel and ion-exchange chromatography on a CM 52 column. 22 of the first 24 N-terminal amino acids were determined and showed 83% and 71% identity to those of human and rat cathepsin G, respectively. Total amino-acid composition demonstrated the basic nature of the protein. In an SDS/polyacrylamide-gel electrophoresis the protein showed an Mr of 29400 compared to the Mr of 26800 calculated from the total amino-acid composition. The enzyme was shown to form complexes with α1α2-macroglobulin and arproteinase inhibitor. A specific enzyme-linked immunosorbent assay was developed for the determination of cathepsin G/α proteinase inhibitor complex in dog plasma and tissue fluids. The mean concentration of cathepsin G in normal dog plasma was determined to be 38 μ/l, measured as cathepsin G/α1-proteinase inhibitor complex. When active dog cathepsin G was added to normal dog plasma in vitro, approximately 56% could be measured by the assay. Slow intravenous infusion of a lethal dose of endotoxin in dogs was followed by a marked drop in white blood cell count and thrombocytes and a simultaneous rapid increase in plasma cathepsin G concentration, reaching a maximum level of 150 β/l. Bile-induced experimental pancreatitis in dogs was accompanied by successive increase in cathepsin G levels in plasma as well as in peritoneal exudates, reaching a maximum level of about 300 μ/l in plasma and 18 mg/ l in the exudates during the late stages of disease. & by Walter de Gruyter & Co
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Axelsson, LenaLund University,Lunds universitet,Experimentell patologi, Malmö,Forskargrupper vid Lunds universitet,Experimental Pathology, Malmö,Lund University Research Groups,Skåne University Hospital(Swepub:lu)expp-lax
(författare)
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Ohlsson, Kjell
(författare)
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Institutionen för kliniska vetenskaper, MalmöMedicinska fakulteten
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Biological Chemistry Hoppe-Seyler: Walter de Gruyter GmbH372:1, s. 419-4260177-3593
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