Kinetic analysis of recombinant antibody-antigen interactions : Relation between structural domains and antigen binding

Borrebaeck, Carl A KLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH (författare)

The relation between domain structures of recombinant monoclonal antibody fragments and their reaction kinetics was studied for the first time using a novel biosensor based on surface plasmon resonance technology. The association and dissociation rate constants of Fab, Fv and single domain (VH fragment) anti-lysozyme antibodies were determined and compared to the intact monoclonal antibody. Fab and Fv fragments showed similar reaction kinetics and had affinity constants of 6 X 109 M-1 and 25 X 109 M-1, respectively. The single domain antibody had significantly different reaction kinetics compared to the fragments consisting of paired heavy and light chain domains. The VH domain had both a higher dissociation and a lower association rate constant, which resulted in an affinity constant approximately 250 times lower than the Fab fragment. This rapid evaluation of antibody reaction kinetics should prove to be an important selection parameter when comparing antibody fragments for their utility in therapeutic or other applications.

Malmborg Hager, Ann-ChristinLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)immt-acm (författare)
Furebring, ChristinaLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)immt-cfu (författare)
Michaelsson, Anne (författare)
Ward, SallyUniversity of Texas (författare)
Danielsson, LenaLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)med-ldn (författare)
Ohlin, MatsLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)immt-moh (författare)
Institutionen för immunteknologiInstitutioner vid LTH (creator_code:org_t)

Ingår i:Bio/Technology: Springer Science and Business Media LLC10:6, s. 697-6980733-222X
Ingår i:Nature Biotechnology: Springer Science and Business Media LLC10:6, s. 697-6981087-01561546-1696

Av författaren/redakt...: Borrebaeck, Carl ...; Malmborg Hager, ...; Furebring, Chris ...; Michaelsson, Ann ...; Ward, Sally; Danielsson, Lena; visa fler...; Ohlin, Mats; visa färre...

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