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Selective cytokine ...
Selective cytokine production by epithelial cells following exposure to Escherichia coli
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- Agace, W. (författare)
- Lund University,Lunds universitet,Slemhinnans immunologi,Forskargrupper vid Lunds universitet,Medicinsk mikrobiologi,Mucosal Immunology,Lund University Research Groups,Medical Microbiology
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- Hedges, S. (författare)
- Lund University
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- Andersson, U. (författare)
- Lund University,Lunds universitet,Medicinsk mikrobiologi,Forskargrupper vid Lunds universitet,Medical Microbiology,Lund University Research Groups
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visa fler...
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Andersson, J. (författare)
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- Ceska, M. (författare)
- Lund University
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- Svanborg, C. (författare)
- Lund University,Lunds universitet,Avdelningen för mikrobiologi, immunologi och glykobiologi - MIG,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Microbiology, Immunology and Glycobiology - MIG,Department of Laboratory Medicine,Faculty of Medicine
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visa färre...
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(creator_code:org_t)
- 1993
- 1993
- Engelska.
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Ingår i: Infection and Immunity. - 0019-9567. ; 61:2, s. 602-609
- Relaterad länk:
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https://lup.lub.lu.s...
Abstract
Ämnesord
Stäng
- This study compared the repertoire of cytokines produced by epithelial cell lines and human peripheral blood monocytes in response to Escherichia coli. The A-498 and J82 urinary tract epithelial cell lines and human peripheral blood monocytes were exposed to E. coli Hu734. The cytokine content of single cells was detected by indirect immunofluorescence using monoclonal antibodies to interleukin-1α (IL-1α), IL-1β, tumor necrosis factor alpha (TNF-α), TNF-β, IL-6, IL-8, and granulocyte macrophage- colony-stimulating factor, and the number of positive cells was used to quantitate the response. The J82 bladder cell line stained positive for IL- 6, IL-8, and IL-1α. The IL-8 and IL-6 response peaked at 2 h, while the number of IL-1α-positive cells reached a peak 6 h after E. coli stimulation. The A-498 kidney cell line stained for IL-8 with a peak at 2 h and IL-6 with a peak at 6 h after E. coli stimulation. Peripheral blood monocytes stained for the cytokines IL-1α, IL-1β, IL-8, IL-6, and TNF-α but not for TNF-β and granulocyte macrophage-colony-stimulating factor after stimulation with E. coli. The results demonstrated that bacteria activated a cytokine response in the epithelial cell lines and monocytes. The epithelial cell lines had a more limited cytokine response profile than circulating monocytes, which may serve to limit the consequences of microbial exposure at the mucosal surface and help maintain the integrity of other tissue compartments.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
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