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Single-Cell Analysi...
Single-Cell Analysis Reveals a Close Relationship between Differentiating Dopamine and Subthalamic Nucleus Neuronal Lineages
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- Kee, Nigel (author)
- Karolinska Institutet,Karolinska Institute,Ludwig Inst Canc Res, Box 240, S-17177 Stockholm, Sweden.;Karolinska Inst, Dept Cell & Mol Biol, S-17177 Stockholm, Sweden.
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- Volakakis, Nikolaos (author)
- Ludwig Institute for Cancer Research, Uppsala branch,Ludwig Inst Canc Res, Box 240, S-17177 Stockholm, Sweden.
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- Kirkeby, Agnete (author)
- Lund University,Lunds universitet,Human neural utvecklingsbiologi,Forskargrupper vid Lunds universitet,Human Neural Developmental Biology,Lund University Research Groups,Lund Univ, Dept Expt Med Sci, Lund Stem Cell Ctr, S-22184 Lund, Sweden.
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- Dahl, Lina (author)
- Ludwig Institute for Cancer Research, Uppsala branch,Ludwig Inst Canc Res, Box 240, S-17177 Stockholm, Sweden.
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- Storvall, Helena (author)
- Ludwig Institute for Cancer Research, Uppsala branch,Ludwig Inst Canc Res, Box 240, S-17177 Stockholm, Sweden.
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- Nolbrant, Sara (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups,Lund Univ, Dept Expt Med Sci, Lund Stem Cell Ctr, S-22184 Lund, Sweden.
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- Lahti, Laura (author)
- Ludwig Institute for Cancer Research, Uppsala branch,Ludwig Inst Canc Res, Box 240, S-17177 Stockholm, Sweden.
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- Björklund, Åsa K. (author)
- Uppsala universitet,Uppsala University,Molekylär evolution,Science for Life Laboratory, SciLifeLab
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- Gillberg, Linda (author)
- Ludwig Institute for Cancer Research, Uppsala branch,Ludwig Inst Canc Res, Box 240, S-17177 Stockholm, Sweden.
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- Joodmardi, Eliza (author)
- Ludwig Institute for Cancer Research, Uppsala branch,Ludwig Inst Canc Res, Box 240, S-17177 Stockholm, Sweden.
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- Sandberg, Rickard (author)
- Karolinska Institutet,Karolinska Institute,Ludwig Institute for Cancer Research, Uppsala branch,Ludwig Inst Canc Res, Box 240, S-17177 Stockholm, Sweden.;Karolinska Inst, Dept Cell & Mol Biol, S-17177 Stockholm, Sweden.
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- Parmar, Malin (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups,Lund Univ, Dept Expt Med Sci, Lund Stem Cell Ctr, S-22184 Lund, Sweden.
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- Perlmann, Thomas (author)
- Karolinska Institutet,Karolinska Institute,Ludwig Institute for Cancer Research, Uppsala branch,Ludwig Inst Canc Res, Box 240, S-17177 Stockholm, Sweden.;Karolinska Inst, Dept Cell & Mol Biol, S-17177 Stockholm, Sweden.
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Karolinska Institute Ludwig Inst Canc Res, Box 240, S-17177 Stockholm, Sweden;Karolinska Inst, Dept Cell & Mol Biol, S-17177 Stockholm, Sweden. (creator_code:org_t)
- Elsevier BV, 2017
- 2017
- English 12 s.
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In: Cell Stem Cell. - : Elsevier BV. - 1934-5909 .- 1875-9777. ; 20:1, s. 29-40
- Related links:
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http://dx.doi.org/10...
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http://www.cell.com/...
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Abstract
Subject headings
Close
- Stem cell engineering and grafting of mesencephalic dopamine (mesDA) neurons is a promising strategy for brain repair in Parkinson's disease (PD). Refinement of differentiation protocols to optimize this approach will require deeper understanding of mesDA neuron development. Here, we studied this process using transcriptome-wide single-cell RNA sequencing of mouse neural progenitors expressing the mesDA neuron determinant Lmx1a. This approach resolved the differentiation of mesDA and neighboring neuronal lineages and revealed a remarkably close relationship between developing mesDA and subthalamic nucleus (STN) neurons, while also highlighting a distinct transcription factor set that can distinguish between them. While previous hESC mesDA differentiation protocols have relied on markers that are shared between the two lineages, we found that application of these highlighted markers can help to refine current stem cell engineering protocols, increasing the proportion of appropriately patterned mesDA progenitors. Our results, therefore, have important implications for cell replacement therapy in PD.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Keyword
- development
- dopamine
- embryogenesis
- graph clustering
- hypothalamus
- neurogenesis
- Parkinson's disease
- single cell RNA-seq
- stem cell
- subthalamic nucleus
Publication and Content Type
- art (subject category)
- ref (subject category)
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To the university's database
- By the author/editor
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Kee, Nigel
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Volakakis, Nikol ...
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Kirkeby, Agnete
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Dahl, Lina
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Storvall, Helena
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Nolbrant, Sara
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show more...
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Lahti, Laura
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Björklund, Åsa K ...
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Gillberg, Linda
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Joodmardi, Eliza
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Sandberg, Rickar ...
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Parmar, Malin
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Perlmann, Thomas
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Neurology
- Articles in the publication
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Cell Stem Cell
- By the university
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Lund University
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Uppsala University
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Karolinska Institutet