Chromosome-level genome assembly of Lilford's wall lizard, Podarcis lilfordi (Günther, 1874) from the Balearic Islands (Spain)

The Mediterranean lizard Podarcis lilfordi is an emblematic species of the Balearic Islands. The extensive phenotypic diversity among extant isolated populations makes the species a great insular model system for eco-evolutionary studies, as well as a challenging target for conservation management plans. Here we report the first high-quality chromosome-level assembly and annotation of the P. lilfordi genome, along with its mitogenome, based on a mixed sequencing strategy (10X Genomics linked reads, Oxford Nanopore Technologies long reads and Hi-C scaffolding) coupled with extensive transcriptomic data (Illumina and PacBio). The genome assembly (1.5 Gb) is highly contiguous (N50 = 90 Mb) and complete, with 99% of the sequence assigned to candidate chromosomal sequences and >97% gene completeness. We annotated a total of 25,663 protein-coding genes translating into 38,615 proteins. Comparison to the genome of the related species Podarcis muralis revealed substantial similarity in genome size, annotation metrics, repeat content, and a strong collinearity, despite their evolutionary distance (∼18-20 MYA). This genome expands the repertoire of available reptilian genomes and will facilitate the exploration of the molecular and evolutionary processes underlying the extraordinary phenotypic diversity of this insular species, while providing a critical resource for conservation genomics.

Cruz, FernandoCenter for Genomic Regulation (CRG) (author)
Alioto, Tyler S.Center for Genomic Regulation (CRG) (author)
Feiner, NathalieLund University,Lunds universitet,Evolutionär ekologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Evolutionsbiologi,Forskargrupper vid Lunds universitet,Evolutionary ecology,Department of Biology,Faculty of Science,Evolutionary Biology,Lund University Research Groups(Swepub:lu)biol-naf (author)
Uller, TobiasLund University,Lunds universitet,Evolutionär ekologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Evolutionsbiologi,Forskargrupper vid Lunds universitet,Evolutionary ecology,Department of Biology,Faculty of Science,Evolutionary Biology,Lund University Research Groups(Swepub:lu)biol-tou (author)
Gut, MartaPompeu Fabra University,Center for Genomic Regulation (CRG) (author)
Sanchez Escudero, IgnacioCenter for Genomic Regulation (CRG) (author)
Tavecchia, GiacomoCSIC Spanish National Research Council (author)
Rotger, AndreuCSIC Spanish National Research Council (author)
Otalora Acevedo, Katherin ElianaUniversity of Barcelona (author)
Baldo, LauraUniversity of Barcelona (author)
Center for Genomic Regulation (CRG)Evolutionär ekologi (creator_code:org_t)

By the author/editor: Gomez-Garrido, J ...; Cruz, Fernando; Alioto, Tyler S.; Feiner, Nathalie; Uller, Tobias; Gut, Marta; show more...; Sanchez Escudero ...; Tavecchia, Giaco ...; Rotger, Andreu; Otalora Acevedo, ...; Baldo, Laura; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Evolutionary Bio ...

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