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Stress induced biof...
Stress induced biofilm formation in Propionibacterium acidipropionici and use in propionic acid production.
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- Cavero Olguin, Victor Hugo (author)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Higher University of San Andrés
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- Hatti-Kaul, Rajni (author)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Cardenas, Oscar Victor (author)
- Higher University of San Andrés
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- Valverde, Marcelo Gutierrez (author)
- Higher University of San Andrés
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- Romero, Danitza Xiomara (author)
- Higher University of San Andrés
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- Alvarez-Aliaga, Maria Teresa (author)
- Higher University of San Andrés
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(creator_code:org_t)
- 2019-06-24
- 2019
- English 12 s.
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In: World Journal of Microbiology and Biotechnology. - : Springer Science and Business Media LLC. - 0959-3993 .- 1573-0972. ; 35:101
- Related links:
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http://dx.doi.org/10...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Propionibacterium acidipropionici produces propionic acid from different sugars and glycerol; the production can be improved by high cell density fermentations using immobilized cells that help to overcome the limitations of the non-productive lag phase and product inhibition. In this study, the use of stress factors to induce P. acidipropionici to form biofilm and its use as an immobilization procedure in fermentations in bioreactors for producing propionic acid was investigated. Citric acid and sodium chloride increased exopolysaccharide production, biofilm capacity index and trehalose production. Analysis of the expression of trehalose synthesis-related genes otsA and treY by RT-qPCR showed significantly increased expression of only treY during log phase with citric acid, while FISH analysis showed expression of treY and luxS under the influence of both stress factors. The stress factors were then used for development of microbial biofilms as immobilization procedure on Poraver® and AnoxKaldnes® carriers in recycle batch reactors for propionic acid production from 20 g/L glycerol. Highest productivities of 0.7 and 0.78 g/L/h were obtained in Poraver® reactors, and 0.39 and 0.43 g/L/h in AnoxKaldnes® reactors with citric acid and NaCl, respectively.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Keyword
- Biofilm; immobilization; propionic acid; stress factor; trehalose
Publication and Content Type
- art (subject category)
- ref (subject category)
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