Search: id:"swepub:oai:lup.lub.lu.se:472cd146-c1b9-4950-9016-ee935d86ee0a" >
In vitro toxicity o...
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Wieslander, Anders P
(author)
In vitro toxicity of biomaterials determined with cell density, total protein, cell cycle distribution and adenine nucleotides
- Article/chapterEnglish1993
Publisher, publication year, extent ...
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2009-07-11
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Informa UK Limited,1993
Numbers
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LIBRIS-ID:oai:lup.lub.lu.se:472cd146-c1b9-4950-9016-ee935d86ee0a
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https://lup.lub.lu.se/record/1107064URI
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https://doi.org/10.3109/10731199309118297DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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Inhibition of cell growth is the most commonly used endpoint for in vitro toxicity of biomaterials. The use of several different endpoints might however generate more information concerning the nature of the toxicity. Thus, we examined the toxicity of two biomaterials, Polyvinylchloride (PVC) and Polyoximethene (POM), with different selected endpoints. The influence of cell growth on these endpoints was also investigated. Water extracts from the polymeric materials were tested on the continuous cell line L-929. Cell density, total protein, total protein per cell, fraction of cells in G0/G1- or S-phase, the concentration of ATP, ADP and AMP were used as endpoints. The PVC material did not significantly influence any of these endpoints until after 72 hours of exposure and the main part of the toxicity at 72 hours was related to higher proliferation rate in control cultures. After the cells had been incubated for 8 hour with POM the main toxic effect was on the energy parameters. In conclusion the PVC material was less toxic than the POM material. Our results also implies that the choice of endpoint will influence the evaluation of cytotoxicity.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Nordin, Marika K
(author)
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Hansson, Björn
(author)
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Baldetorp, BoLund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)onk-bba
(author)
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Kjellstrand, Per T T
(author)
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Bröstcancer-genetikSektion I
(creator_code:org_t)
Related titles
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In:Artificial Cells, Blood Substitutes, and Biotechnology: Informa UK Limited21:1, s. 63-701055-7172
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