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A multicentric stud...
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Vialas, VitalComplutense University of Madrid
(author)
A multicentric study to evaluate the use of relative retention times in targeted proteomics
- Article/chapterEnglish2017
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LIBRIS-ID:oai:lup.lub.lu.se:47fc4f1e-2c18-4477-8507-2504703b8b7e
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https://lup.lub.lu.se/record/47fc4f1e-2c18-4477-8507-2504703b8b7eURI
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https://doi.org/10.1016/j.jprot.2016.10.014DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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Despite the maturity reached by targeted proteomic strategies, reliable and standardized protocols are urgently needed to enhance reproducibility among different laboratories and analytical platforms, facilitating a more widespread use in biomedical research. To achieve this goal, the use of dimensionless relative retention times (iRT), defined on the basis of peptide standard retention times (RT), has lately emerged as a powerful tool. The robustness, reproducibility and utility of this strategy were examined for the first time in a multicentric setting, involving 28 laboratories that included 24 of the Spanish network of proteomics laboratories (ProteoRed-ISCIII). According to the results obtained in this study, dimensionless retention time values (iRTs) demonstrated to be a useful tool for transferring and sharing peptide retention times across different chromatographic set-ups both intra- and inter-laboratories. iRT values also showed very low variability over long time periods. Furthermore, parallel quantitative analyses showed a high reproducibility despite the variety of experimental strategies used, either MRM (multiple reaction monitoring) or pseudoMRM, and the diversity of analytical platforms employed. Biological significance From the very beginning of proteomics as an analytical science there has been a growing interest in developing standardized methods and experimental procedures in order to ensure the highest quality and reproducibility of the results. In this regard, the recent (2012) introduction of the dimensionless retention time concept has been a significant advance. In our multicentric (28 laboratories) study we explore the usefulness of this concept in the context of a targeted proteomics experiment, demonstrating that dimensionless retention time values is a useful tool for transferring and sharing peptide retention times across different chromatographic set-ups.
Subject headings and genre
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Colomé-Calls, NúriaVall d'Hebron University Hospital
(author)
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Abian, JoaquínCSIC Institute of Biomedical Research of Barcelona (IIBB)
(author)
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Aloria, KermanUniversity of the Basque Country
(author)
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Alvarez-Llamas, GloriaHospital Fundación Jiménez Díaz
(author)
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Antúnez, OretoUniversity of Valencia
(author)
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Arizmendi, Jesus M.University of the Basque Country
(author)
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Azkargorta, MikelCenter For Cooperative Research In Biosciences (CIC bioGUNE)
(author)
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Barceló-Batllori, SilviaBellvitge Biomedical Research Institute
(author)
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Barderas, María G.Hospital Nacional de Parapléjicos
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Blanco, Francisco JInstitute of Biomedical Research of A Coruña, INIBIC
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Casal, J. IgnacioInstitute of Biomedical Research of A Coruña, INIBIC
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Casas, VanessaInstitute of Biomedical Research of A Coruña, INIBIC
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de la Torre, CarolinaBellvitge Biomedical Research Institute
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Chicano-Gálvez, EduardoMaimonides Biomedical Research Institute of Cordoba,University of Córdoba, Spain
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Elortza, FelixCenter For Cooperative Research In Biosciences (CIC bioGUNE)
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Espadas, GuadalupePompeu Fabra University
(author)
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Estanyol, Josep M.University of Barcelona
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Fernandez-Irigoyen, JoaquínNavarrabiomed
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Fernandez-Puente, PatriciaInstitute of Biomedical Research of A Coruña, INIBIC
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Fidalgo, María JoséUniversity of Barcelona
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Fuentes, ManuelUniversity of Salamanca
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Gay, MarinaInstitute for Research in Biomedicine (IRB Barcelona)
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Gil, ConchaComplutense University of Madrid
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Hainard, AlexandreUniversity of Geneva
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Hernaez, Maria LuisaComplutense University of Madrid
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Ibarrola, NievesUniversity of Salamanca
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Kopylov, Arthur T.VN Orekhovich Scientific Research Institute of Biomedical Chemistry, Russian Academy Of Medical Science
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Lario, AntonioCSIC Institute of Parasitology and Biomedicine Lopez-Neyra (IPBLN)
(author)
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Lopez, Juan AntonioCentro Nacional de Investigaciones Cardiovasculares Carlos III
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López-Lucendo, MaríaBiological Research Center (CIB), Madrid
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Marcilla, MiguelCSIC National Center for Biotechnology (CNB)
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Marina-Ramírez, AnabelCSIC Center of Molecular Biology Severo Ochoa (CBMSO)
(author)
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Marko-Varga, GyorgyLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)akem-gmv
(author)
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Martín, LunaVall d'Hebron University Hospital
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Mora, Maria I.University of Navarra
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Morato-López, EsperanzaCSIC Center of Molecular Biology Severo Ochoa (CBMSO)
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Muñoz, JavierSpanish National Cancer Research Center (CNIO)
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Odena, Maria AntoniaUniversity of Barcelona
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de Oliveira, EliandreUniversity of Barcelona
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Orera, IreneAragon Health Sciences Institute
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Ortea, IgnacioUniversity of Córdoba, Spain
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Pasquarello, CarlaUniversity of Geneva
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Ray, Kevin B.MilliporeSigma
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Rezeli, MelindaLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)elma-mrl
(author)
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Ruppen, IsabelSpanish National Cancer Research Center (CNIO)
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Sabidó, EduardPompeu Fabra University
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del Pino, Manuel M SanchezUniversity of Valencia
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Sancho, JaimeCSIC Institute of Parasitology and Biomedicine Lopez-Neyra (IPBLN)
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Santamaría, EnriqueNavarrabiomed
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Vazquez, JesusCentro Nacional de Investigaciones Cardiovasculares Carlos III
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Vilaseca, MartaInstitute for Research in Biomedicine (IRB Barcelona)
(author)
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Vivanco, FernandoHospital Fundación Jiménez Díaz
(author)
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Walters, James J.MilliporeSigma
(author)
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Zgoda, Victor G.VN Orekhovich Scientific Research Institute of Biomedical Chemistry, Russian Academy Of Medical Science
(author)
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Corrales, Fernando J.University of Navarra
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Canals, FrancescVall d'Hebron University Hospital
(author)
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Paradela, AlbertoCSIC National Center for Biotechnology (CNB)
(author)
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Complutense University of MadridVall d'Hebron University Hospital
(creator_code:org_t)
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In:Journal of Proteomics: Elsevier BV152, s. 138-1491874-3919
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Vialas, Vital
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Colomé-Calls, Nú ...
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Abian, Joaquín
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Aloria, Kerman
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Alvarez-Llamas, ...
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Antúnez, Oreto
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Arizmendi, Jesus ...
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Azkargorta, Mike ...
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Barceló-Batllori ...
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Barderas, María ...
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Blanco, Francisc ...
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Casal, J. Ignaci ...
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Casas, Vanessa
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de la Torre, Car ...
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Chicano-Gálvez, ...
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Elortza, Felix
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Espadas, Guadalu ...
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Estanyol, Josep ...
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Fernandez-Irigoy ...
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Fernandez-Puente ...
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Fidalgo, María J ...
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Fuentes, Manuel
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Gay, Marina
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Gil, Concha
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Hainard, Alexand ...
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Hernaez, Maria L ...
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Ibarrola, Nieves
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Kopylov, Arthur ...
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Lario, Antonio
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Lopez, Juan Anto ...
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López-Lucendo, M ...
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Marcilla, Miguel
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Marina-Ramírez, ...
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Marko-Varga, Gyo ...
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Martín, Luna
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Mora, Maria I.
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Morato-López, Es ...
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Muñoz, Javier
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Odena, Maria Ant ...
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de Oliveira, Eli ...
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Orera, Irene
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Ortea, Ignacio
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Pasquarello, Car ...
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Ray, Kevin B.
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Rezeli, Melinda
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Ruppen, Isabel
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Sabidó, Eduard
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del Pino, Manuel ...
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Sancho, Jaime
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Santamaría, Enri ...
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Vazquez, Jesus
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Vilaseca, Marta
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Vivanco, Fernand ...
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Walters, James J ...
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Zgoda, Victor G.
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Corrales, Fernan ...
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Canals, Francesc
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Paradela, Albert ...
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Journal of Prote ...
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Lund University