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A multicentric stud...
A multicentric study to evaluate the use of relative retention times in targeted proteomics
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- Vialas, Vital (author)
- Complutense University of Madrid
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- Colomé-Calls, Núria (author)
- Vall d'Hebron University Hospital
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- Abian, Joaquín (author)
- CSIC Institute of Biomedical Research of Barcelona (IIBB)
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- Aloria, Kerman (author)
- University of the Basque Country
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- Alvarez-Llamas, Gloria (author)
- Hospital Fundación Jiménez Díaz
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- Antúnez, Oreto (author)
- University of Valencia
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- Arizmendi, Jesus M. (author)
- University of the Basque Country
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- Azkargorta, Mikel (author)
- Center For Cooperative Research In Biosciences (CIC bioGUNE)
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- Barceló-Batllori, Silvia (author)
- Bellvitge Biomedical Research Institute
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- Barderas, María G. (author)
- Hospital Nacional de Parapléjicos
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- Blanco, Francisco J (author)
- Institute of Biomedical Research of A Coruña, INIBIC
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- Casal, J. Ignacio (author)
- Institute of Biomedical Research of A Coruña, INIBIC
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- Casas, Vanessa (author)
- Institute of Biomedical Research of A Coruña, INIBIC
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- de la Torre, Carolina (author)
- Bellvitge Biomedical Research Institute
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- Chicano-Gálvez, Eduardo (author)
- Maimonides Biomedical Research Institute of Cordoba,University of Córdoba, Spain
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- Elortza, Felix (author)
- Center For Cooperative Research In Biosciences (CIC bioGUNE)
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- Espadas, Guadalupe (author)
- Pompeu Fabra University
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- Estanyol, Josep M. (author)
- University of Barcelona
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- Fernandez-Irigoyen, Joaquín (author)
- Navarrabiomed
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- Fernandez-Puente, Patricia (author)
- Institute of Biomedical Research of A Coruña, INIBIC
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- Fidalgo, María José (author)
- University of Barcelona
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- Fuentes, Manuel (author)
- University of Salamanca
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- Gay, Marina (author)
- Institute for Research in Biomedicine (IRB Barcelona)
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- Gil, Concha (author)
- Complutense University of Madrid
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- Hainard, Alexandre (author)
- University of Geneva
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- Hernaez, Maria Luisa (author)
- Complutense University of Madrid
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- Ibarrola, Nieves (author)
- University of Salamanca
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- Kopylov, Arthur T. (author)
- VN Orekhovich Scientific Research Institute of Biomedical Chemistry, Russian Academy Of Medical Science
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- Lario, Antonio (author)
- CSIC Institute of Parasitology and Biomedicine Lopez-Neyra (IPBLN)
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- Lopez, Juan Antonio (author)
- Centro Nacional de Investigaciones Cardiovasculares Carlos III
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- López-Lucendo, María (author)
- Biological Research Center (CIB), Madrid
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- Marcilla, Miguel (author)
- CSIC National Center for Biotechnology (CNB)
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- Marina-Ramírez, Anabel (author)
- CSIC Center of Molecular Biology Severo Ochoa (CBMSO)
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- Marko-Varga, Gyorgy (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Martín, Luna (author)
- Vall d'Hebron University Hospital
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- Mora, Maria I. (author)
- University of Navarra
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- Morato-López, Esperanza (author)
- CSIC Center of Molecular Biology Severo Ochoa (CBMSO)
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- Muñoz, Javier (author)
- Spanish National Cancer Research Center (CNIO)
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- Odena, Maria Antonia (author)
- University of Barcelona
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- de Oliveira, Eliandre (author)
- University of Barcelona
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- Orera, Irene (author)
- Aragon Health Sciences Institute
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- Ortea, Ignacio (author)
- University of Córdoba, Spain
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- Pasquarello, Carla (author)
- University of Geneva
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- Ray, Kevin B. (author)
- MilliporeSigma
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- Rezeli, Melinda (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Ruppen, Isabel (author)
- Spanish National Cancer Research Center (CNIO)
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- Sabidó, Eduard (author)
- Pompeu Fabra University
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- del Pino, Manuel M Sanchez (author)
- University of Valencia
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- Sancho, Jaime (author)
- CSIC Institute of Parasitology and Biomedicine Lopez-Neyra (IPBLN)
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- Santamaría, Enrique (author)
- Navarrabiomed
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- Vazquez, Jesus (author)
- Centro Nacional de Investigaciones Cardiovasculares Carlos III
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- Vilaseca, Marta (author)
- Institute for Research in Biomedicine (IRB Barcelona)
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- Vivanco, Fernando (author)
- Hospital Fundación Jiménez Díaz
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- Walters, James J. (author)
- MilliporeSigma
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- Zgoda, Victor G. (author)
- VN Orekhovich Scientific Research Institute of Biomedical Chemistry, Russian Academy Of Medical Science
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- Corrales, Fernando J. (author)
- University of Navarra
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- Canals, Francesc (author)
- Vall d'Hebron University Hospital
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- Paradela, Alberto (author)
- CSIC National Center for Biotechnology (CNB)
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(creator_code:org_t)
- Elsevier BV, 2017
- 2017
- English 12 s.
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In: Journal of Proteomics. - : Elsevier BV. - 1874-3919. ; 152, s. 138-149
- Related links:
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http://dx.doi.org/10...
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https://repisalud.is...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Despite the maturity reached by targeted proteomic strategies, reliable and standardized protocols are urgently needed to enhance reproducibility among different laboratories and analytical platforms, facilitating a more widespread use in biomedical research. To achieve this goal, the use of dimensionless relative retention times (iRT), defined on the basis of peptide standard retention times (RT), has lately emerged as a powerful tool. The robustness, reproducibility and utility of this strategy were examined for the first time in a multicentric setting, involving 28 laboratories that included 24 of the Spanish network of proteomics laboratories (ProteoRed-ISCIII). According to the results obtained in this study, dimensionless retention time values (iRTs) demonstrated to be a useful tool for transferring and sharing peptide retention times across different chromatographic set-ups both intra- and inter-laboratories. iRT values also showed very low variability over long time periods. Furthermore, parallel quantitative analyses showed a high reproducibility despite the variety of experimental strategies used, either MRM (multiple reaction monitoring) or pseudoMRM, and the diversity of analytical platforms employed. Biological significance From the very beginning of proteomics as an analytical science there has been a growing interest in developing standardized methods and experimental procedures in order to ensure the highest quality and reproducibility of the results. In this regard, the recent (2012) introduction of the dimensionless retention time concept has been a significant advance. In our multicentric (28 laboratories) study we explore the usefulness of this concept in the context of a targeted proteomics experiment, demonstrating that dimensionless retention time values is a useful tool for transferring and sharing peptide retention times across different chromatographic set-ups.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Inter-laboratory validation
- Liquid chromatography
- Multiple reaction monitoring
- Proteomics
- Reproducibility
- Standardization
- Targeted proteomics
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Vialas, Vital
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Colomé-Calls, Nú ...
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Abian, Joaquín
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Aloria, Kerman
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Alvarez-Llamas, ...
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Antúnez, Oreto
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show more...
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Arizmendi, Jesus ...
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Azkargorta, Mike ...
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Barceló-Batllori ...
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Barderas, María ...
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Blanco, Francisc ...
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Casal, J. Ignaci ...
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Casas, Vanessa
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de la Torre, Car ...
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Chicano-Gálvez, ...
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Elortza, Felix
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Espadas, Guadalu ...
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Estanyol, Josep ...
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Fernandez-Irigoy ...
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Fernandez-Puente ...
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Fidalgo, María J ...
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Fuentes, Manuel
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Gay, Marina
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Gil, Concha
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Hainard, Alexand ...
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Hernaez, Maria L ...
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Ibarrola, Nieves
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Kopylov, Arthur ...
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Lario, Antonio
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Lopez, Juan Anto ...
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López-Lucendo, M ...
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Marcilla, Miguel
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Marina-Ramírez, ...
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Marko-Varga, Gyo ...
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Martín, Luna
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Mora, Maria I.
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Morato-López, Es ...
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Muñoz, Javier
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Odena, Maria Ant ...
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de Oliveira, Eli ...
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Orera, Irene
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Ortea, Ignacio
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Pasquarello, Car ...
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Ray, Kevin B.
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Rezeli, Melinda
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Ruppen, Isabel
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Sabidó, Eduard
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del Pino, Manuel ...
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Sancho, Jaime
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Santamaría, Enri ...
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Vazquez, Jesus
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Vilaseca, Marta
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Vivanco, Fernand ...
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Walters, James J ...
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Zgoda, Victor G.
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Corrales, Fernan ...
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Canals, Francesc
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Paradela, Albert ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Journal of Prote ...
- By the university
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Lund University