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Minimal residual di...
Minimal residual disease assessment in childhood acute lymphoblastic leukaemia: a Swedish multi-centre study comparing real-time polymerase chain reaction and multicolour flow cytometry.
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- Thörnerup, Ingrid (author)
- Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Department of Clinical Sciences, Malmö,Faculty of Medicine
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- Forestier, Erik (author)
- Umeå universitet,Medicinsk och klinisk genetik,Pediatrik,Department of Clinical Sciences, Paediatrics, Umeå University, Umeå
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- Botling, Johan (author)
- Uppsala universitet,Molekylär och morfologisk patologi,Forskargrupp Botling,Uppsala University
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- Thuresson, Britt (author)
- Lund University,Lunds universitet,Avdelningen för hematologi och transfusionsmedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Hematology and Transfusion Medicine,Department of Laboratory Medicine,Faculty of Medicine
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- Wasslavik, Carina (author)
- Sahlgrens University Hospital
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- Björklund, Elisabet (author)
- Karolinska University Hospital
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- Li, Aihong (author)
- Umeå universitet,Klinisk kemi,Umea University
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- Lindström-Eriksson, Eleonor (author)
- Umea University
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- Malec, Maria (author)
- Karolinska University Hospital
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- Grönlund, Elisabeth (author)
- Umeå universitet,Patologi,Umea University
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- Torikka, Kerstin (author)
- Lund University
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- Heldrup, Jesper (author)
- Lund University,Lunds universitet,Pediatrik, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Paediatrics (Lund),Section V,Department of Clinical Sciences, Lund,Faculty of Medicine,University Lund Hospital
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- Abrahamsson, Jonas, 1954 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics,Sahlgrens University Hospital
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- Behrendtz, Mikael (author)
- Linköpings universitet,Östergötlands Läns Landsting,Barn- och ungdomskliniken i Linköping,Pediatrik,Hälsouniversitetet
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- Söderhäll, Stefan (author)
- Karolinska Institutet,Sahlgrens University Hospital
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- Jacobsson, Stefan, 1951 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
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- Olofsson, Tor (author)
- Lund University,Lunds universitet,Avdelningen för hematologi och transfusionsmedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Hematology and Transfusion Medicine,Department of Laboratory Medicine,Faculty of Medicine
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- Porwit, Anna (author)
- Karolinska Institutet,Karolinska University Hospital
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- Lönnerholm, Gudmar (author)
- Uppsala universitet,Pediatrik,Barnonkologisk forskning/Pfeifer,Uppsala University
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- Rosenquist, Richard (author)
- Uppsala universitet,Hematologi och immunologi,Forskargrupp Rosenquist Brandell,Uppsala University
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- Sundström, Christer (author)
- Uppsala universitet,Molekylär och morfologisk patologi,Forskargrupp Alafuzoff,Uppsala University
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- Thörn, Ingrid (author)
- Uppsala universitet,Molekylär och morfologisk patologi,Forskargrupp Alafuzoff,Uppsala University
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(creator_code:org_t)
- 2011-01-20
- 2011
- English.
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In: British Journal of Haematology. - : Wiley. - 0007-1048 .- 1365-2141. ; 152:6, s. 743-753
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Abstract
Subject headings
Close
- Minimal residual disease (MRD) assessment is a powerful prognostic factor for determining the risk of relapse in childhood acute lymphoblastic leukaemia (ALL). In this Swedish multi-centre study of childhood ALL diagnosed between 2002 and 2006, the MRD levels were analysed in 726 follow-up samples in 228 children using real-time quantitative polymerase chain reaction (RQ-PCR) of rearranged immunoglobulin/T-cell receptor genes and multicolour flow cytometry (FCM). Using an MRD threshold of 0·1%, which was the sensitivity level reached in all analyses, the concordance between RQ-PCR and FCM MRD values at day 29 was 84%. In B-cell precursor ALL, an MRD level of ≥0·1% at day 29 predicted a higher risk of bone marrow relapse (BMR) with both methods, although FCM was a better discriminator. However, considering the higher median MRD values achieved with RQ-PCR, a higher MRD cut-off (≥0·2%) improved the predictive capacity of RQ-PCR. In T-ALL, RQ-PCR was notably superior to FCM in predicting risk of BMR. That notwithstanding, MRD levels of ≥0·1%, detected by either method at day 29, could not predict isolated extramedullary relapse. In conclusion, the concordance between RQ-PCR and FCM was high and hence both methods are valuable clinical tools for identifying childhood ALL cases with increased risk of BMR.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
- SAMHÄLLSVETENSKAP -- Annan samhällsvetenskap -- Övrig annan samhällsvetenskap (hsv//swe)
- SOCIAL SCIENCES -- Other Social Sciences -- Other Social Sciences not elsewhere specified (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- childhood acute lymphoblastic leukaemia
- disease
- minimal residual
- TCR genes
- rearranged IG
- flow cytometry
- RQ-PCR
- flow cytometry
- MEDICINE
- TECHNOLOGY
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Thörnerup, Ingri ...
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Forestier, Erik
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Botling, Johan
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Thuresson, Britt
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Wasslavik, Carin ...
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Björklund, Elisa ...
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Li, Aihong
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Lindström-Erikss ...
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Malec, Maria
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Grönlund, Elisab ...
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Torikka, Kerstin
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Heldrup, Jesper
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Abrahamsson, Jon ...
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Behrendtz, Mikae ...
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Söderhäll, Stefa ...
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Jacobsson, Stefa ...
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Olofsson, Tor
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Porwit, Anna
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Lönnerholm, Gudm ...
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Rosenquist, Rich ...
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Sundström, Chris ...
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Thörn, Ingrid
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and Hematology
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- Articles in the publication
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British Journal ...
- By the university
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Lund University
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Umeå University
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University of Gothenburg
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Uppsala University
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Karolinska Institutet
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Linköping University