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Benchmarking immuno...
Benchmarking immunoinformatic tools for the analysis of antibody repertoire sequences
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- Smakaj, Erand (author)
- University of Applied Sciences and Arts Northwestern Switzerland (FHNW)
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- Babrak, Lmar (author)
- University of Applied Sciences and Arts Northwestern Switzerland (FHNW)
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- Ohlin, Mats (author)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH,LUCC: Lund University Cancer Centre,Other Strong Research Environments
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- Shugay, Mikhail (author)
- Skolkovo Institute of Science and Technology
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- Briney, Bryan (author)
- Scripps Research Institute
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- Tosoni, Deniz (author)
- University of Applied Sciences and Arts Northwestern Switzerland (FHNW)
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- Galli, Christopher (author)
- University of Applied Sciences and Arts Northwestern Switzerland (FHNW)
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- Grobelsek, Vendi (author)
- ETH Zürich
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- D'Angelo, Igor (author)
- Amgen Inc.
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- Olson, Branden (author)
- University of Washington,Fred Hutchinson Cancer Research Center
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- Reddy, Sai (author)
- ETH Zürich
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- Greiff, Victor (author)
- University of Oslo
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- Trück, Johannes (author)
- University of Zurich,Children's University Hospital, Zurich
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- Marquez, Susanna (author)
- Yale University
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- Lees, William (author)
- University of London
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- Miho, Enkelejda (author)
- University of Applied Sciences and Arts Northwestern Switzerland (FHNW),aiNET GmbH
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(creator_code:org_t)
- 2019-12-24
- 2020
- English 9 s.
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In: Bioinformatics. - : Oxford University Press (OUP). - 1367-4803 .- 1460-2059. ; 36:6, s. 1731-1739
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Abstract
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- SUMMARY: Antibody repertoires reveal insights into the biology of the adaptive immune system and empower diagnostics and therapeutics. There are currently multiple tools available for the annotation of antibody sequences. All downstream analyses such as choosing lead drug candidates depend on the correct annotation of these sequences; however, a thorough comparison of the performance of these tools has not been investigated. Here, we benchmark the performance of commonly used immunoinformatic tools, i.e. IMGT/HighV-QUEST, IgBLAST and MiXCR, in terms of reproducibility of annotation output, accuracy and speed using simulated and experimental high-throughput sequencing datasets.We analyzed changes in IMGT reference germline database in the last 10 years in order to assess the reproducibility of the annotation output. We found that only 73/183 (40%) V, D and J human genes were shared between the reference germline sets used by the tools. We found that the annotation results differed between tools. In terms of alignment accuracy, MiXCR had the highest average frequency of gene mishits, 0.02 mishit frequency and IgBLAST the lowest, 0.004 mishit frequency. Reproducibility in the output of complementarity determining three regions (CDR3 amino acids) ranged from 4.3% to 77.6% with preprocessed data. In addition, run time of the tools was assessed: MiXCR was the fastest tool for number of sequences processed per unit of time. These results indicate that immunoinformatic analyses greatly depend on the choice of bioinformatics tool. Our results support informed decision-making to immunoinformaticians based on repertoire composition and sequencing platforms. AVAILABILITY AND IMPLEMENTATION: All tools utilized in the paper are free for academic use. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk laboratorie- och mätteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Laboratory and Measurements Technologies (hsv//eng)
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- art (subject category)
- ref (subject category)
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- By the author/editor
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Smakaj, Erand
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Babrak, Lmar
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Ohlin, Mats
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Shugay, Mikhail
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Briney, Bryan
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Tosoni, Deniz
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show more...
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Galli, Christoph ...
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Grobelsek, Vendi
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D'Angelo, Igor
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Olson, Branden
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Reddy, Sai
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Greiff, Victor
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Trück, Johannes
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Marquez, Susanna
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Lees, William
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Miho, Enkelejda
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- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Medical Engineer ...
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and Medical Laborato ...
- Articles in the publication
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Bioinformatics
- By the university
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Lund University