Chemical Methods for Detection of Allergens and Skin Exposure

• Many allergens are widely used in both consumer and occupational products. In many cases, it is difficult to know all the ingredients of a product since most products are not sufficiently labelled. To diagnose and prevent allergic contact dermatitis, the demonstration of allergens in the products from the patient’s environment is important. Chemical analysis of a product can make it possible to demonstrate the presence or absence of known allergens. Simple spot tests or documented analytical methods such as thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), gas chromatography (GC), atomic absorption spectrophotometry (AAS), and inductively coupled plasma-mass spectrometry (ICP-MS) can be used. Moreover, with chemical methods, the purity of a substance can be checked and new allergens can be isolated and identified. Advanced methods such as mass spectrometry (MS), nuclear magnetic resonance spectroscopy (NMR), and infrared spectrophotometry (IR) are often required to identify isolated allergens.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)

Nyckelord

Acid wipe sampling

Atomic absorption

Bag rinsing

Chemical analysis

Chromate

Cobalt

Epoxy

Extract

Fluorescent tracer

Formaldehyde

Gas chromatography

IR

Liquid chromatography

Mass spectrometry

Nickel

NMR

Skin exposure assessment

Spectrophotometry

Spot test

Surrogate skin sampling

Tape stripping

Thin layer chromatography

UV

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