Sökning: onr:"swepub:oai:lup.lub.lu.se:53856e88-a0a7-4f40-bd71-908794be95d6" > IAP-Based Cell Sort...
Fältnamn | Indikatorer | Metadata |
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000 | 05135naa a2200577 4500 | |
001 | oai:lup.lub.lu.se:53856e88-a0a7-4f40-bd71-908794be95d6 | |
003 | SwePub | |
008 | 171010s2017 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/53856e88-a0a7-4f40-bd71-908794be95d62 URI |
024 | 7 | a https://doi.org/10.1016/j.stemcr.2017.08.0162 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Lehnen, Danielau Miltenyi Biotec GmbH4 aut |
245 | 1 0 | a IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells |
264 | 1 | b Elsevier BV,c 2017 |
520 | a Human pluripotent stem cell (hPSC)-derived mesencephalic dopaminergic (mesDA) neurons can relieve motor deficits in animal models of Parkinson's disease (PD). Clinical translation of differentiation protocols requires standardization of production procedures, and surface-marker-based cell sorting is considered instrumental for reproducible generation of defined cell products. Here, we demonstrate that integrin-associated protein (IAP) is a cell surface marker suitable for enrichment of hPSC-derived mesDA progenitor cells. Immunomagnetically sorted IAP+ mesDA progenitors showed increased expression of ventral midbrain floor plate markers, lacked expression of pluripotency markers, and differentiated into mature dopaminergic (DA) neurons in vitro. Intrastriatal transplantation of IAP+ cells sorted at day 16 of differentiation in a rat model of PD resulted in functional recovery. Grafts from sorted IAP+ mesDA progenitors were more homogeneous in size and DA neuron density. Thus, we suggest IAP-based sorting for reproducible prospective enrichment of mesDA progenitor cells in clinical cell replacement strategies. In this article, Knöbel and colleagues identified IAP as a cell surface marker for mesDA progenitor cells. Immunomagnetic sorting for IAP+ led to reproducible and homogeneous cell compositions. Intrastriatal transplantation of sorted cells at day 16 of differentiation in a PD rat model resulted in functional recovery, and grafts were more homogeneous in size and DA neuron density than unsorted cells. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Neurovetenskaper0 (SwePub)301052 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Neurosciences0 (SwePub)301052 hsv//eng |
653 | a CD47 | |
653 | a Cell replacement therapy | |
653 | a Dopaminergic | |
653 | a Floor plate | |
653 | a IAP | |
653 | a Immunomagnetic sorting (MACS) | |
653 | a MesDA progenitor cells | |
653 | a Midbrain | |
653 | a Parkinson's disease | |
653 | a PD | |
653 | a Regenerative medicine | |
700 | 1 | a Barral, Serenau Aix-Marseille University,University College London4 aut |
700 | 1 | a Cardoso, Tiagou Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups4 aut0 (Swepub:lu)med-tcd |
700 | 1 | a Grealish, Shaneu Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups4 aut0 (Swepub:lu)med-sge |
700 | 1 | a Heuer, Andreasu Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine4 aut0 (Swepub:lu)med-ah4 |
700 | 1 | a Smiyakin, Andreju Miltenyi Biotec GmbH4 aut |
700 | 1 | a Kirkeby, Agneteu Lund University,Lunds universitet,Human neural utvecklingsbiologi,Forskargrupper vid Lunds universitet,Human Neural Developmental Biology,Lund University Research Groups4 aut0 (Swepub:lu)med-agk |
700 | 1 | a Kollet, Juttau Miltenyi Biotec GmbH4 aut |
700 | 1 | a Cremer, Haroldu Aix-Marseille University4 aut |
700 | 1 | a Parmar, Malinu Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups4 aut0 (Swepub:lu)mphy-mpa |
700 | 1 | a Bosio, Andreasu Miltenyi Biotec GmbH4 aut |
700 | 1 | a Knöbel, Sebastianu Miltenyi Biotec GmbH4 aut |
710 | 2 | a Miltenyi Biotec GmbHb Aix-Marseille University4 org |
773 | 0 | t Stem Cell Reportsd : Elsevier BVg 9:4, s. 1207-1220q 9:4<1207-1220x 2213-6711 |
856 | 4 | u http://dx.doi.org/10.1016/j.stemcr.2017.08.016x freey FULLTEXT |
856 | 4 | u http://www.cell.com/article/S2213671117303739/pdf |
856 | 4 8 | u https://lup.lub.lu.se/record/53856e88-a0a7-4f40-bd71-908794be95d6 |
856 | 4 8 | u https://doi.org/10.1016/j.stemcr.2017.08.016 |
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