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Selective protein a...
Selective protein aggregation confines and inhibits endotoxins in wounds : Linking host defense to amyloid formation
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- Petrlova, Jitka (författare)
- Lund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, 22184 Lund, Sweden
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- Hartman, Erik (författare)
- Skåne University Hospital,Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, 22184 Lund, Sweden
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- Petruk, Ganna (författare)
- Lund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, 22184 Lund, Sweden
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- Lim, Jeremy Chun Hwee (författare)
- Lund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, 22184 Lund, Sweden
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- Adav, Sunil Shankar (författare)
- School of Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore
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- Kjellström, Sven (författare)
- Lund University,Lunds universitet,Masspektrometri,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,BioMS,Forskargrupper vid Lunds universitet,Mass Spectrometry,Section V,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University Research Groups,Department of Clinical Sciences, BioMS, Lund University, Lund, Sweden
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- Puthia, Manoj (författare)
- Lund University,Lunds universitet,Avdelningen för mikrobiologi, immunologi och glykobiologi - MIG,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Microbiology, Immunology and Glycobiology - MIG,Department of Laboratory Medicine,Faculty of Medicine,Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, 22184 Lund, Sweden
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- Schmidtchen, Artur (författare)
- Lund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, 22184 Lund, Sweden Dermatology, Skane University Hospital, 22185 Lund, Sweden
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(creator_code:org_t)
- Elsevier BV, 2023
- 2023
- Engelska.
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Ingår i: iScience. - : Elsevier BV. - 2589-0042. ; 26:10
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http://dx.doi.org/10... (free)
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https://mau.diva-por... (primary) (Raw object)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Bacterial lipopolysaccharide (LPS) induces rapid protein aggregation in human wound fluid. We aimed to characterize these LPS-induced aggregates and their functional implications using a combination of mass spectrometry analyses, biochemical assays, biological imaging, cell experiments, and animal models. The wound-fluid aggregates encompass diverse protein classes, including sequences from coagulation factors, annexins, histones, antimicrobial proteins/peptides, and apolipoproteins. We identified proteins and peptides with a high aggregation propensity and verified selected components through Western blot analysis. Thioflavin T and Amytracker staining revealed amyloid-like aggregates formed after exposure to LPS in vitro in human wound fluid and in vivo in porcine wound models. Using NF-κB-reporter mice and IVIS bioimaging, we demonstrate that such wound-fluid LPS aggregates induce a significant reduction in local inflammation compared with LPS in plasma. The results show that protein/peptide aggregation is a mechanism for confining LPS and reducing inflammation, further emphasizing the connection between host defense and amyloidogenesis.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
Nyckelord
- Bacteriology
- cell biology
- Immunology
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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