Clonal chromosome aberrations are present in vivo in synovia and osteophytes from patients with osteoarthritis

We have previously reported recurrent clonal chromosomal aberrations in synovia, osteophytes and articular cartilage from patients with osteoarthritis (OA). In particular, gain of chromosomes 5 and 7 was found to be strongly associated with OA. In order to exclude the possibility of in vitro artefacts, we studied three to four parallel, independent cultures from ten samples of synovia and three samples of osteophytes from ten women with primary OA. In all, 40 cultures were cytogenetically analysed, 39 of which had clonal chromosomal aberrations. The most common aberrations were +7 and +5 which were found in 38 and 12 cultures, respectively. There were striking karyotype similarities among the parallel cultures from each case. Out of a total of 83 clones, only 11 were unique for one culture, 7 from synovia and 4 from osteophytes. The genetic homogeneity among different cultures from the same patients excludes the possibility of in vitro artefacts and indicates a widespread distribution of the cytogenetically aberrant clones in vivo.

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Reumatologi och inflammation hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Rheumatology and Autoimmunity hsv//eng
Aged
Aged, 80 and over
Bone and Bones/pathology
Chromosome Aberrations
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 7
Clone Cells
Cytogenetics
Female
Humans
Karyotyping
Knee Joint/pathology
Osteoarthritis/genetics
Osteoblasts/pathology
Synovial Membrane/pathology

Limon, J (författare)
Pålsson, ESkåne University Hospital(Swepub:lu)kgen-epa (författare)
Lindstrand, ASkåne University Hospital(Swepub:lu)ort-ali (författare)
Toksvig-Larsen, SSkåne University Hospital(Swepub:lu)ort-stl (författare)
Mandahl, NLund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine,Skåne University Hospital(Swepub:lu)kgen-nma (författare)
Mertens, FSkåne University Hospital(Swepub:lu)kgen-fme (författare)
Skåne University HospitalAvdelningen för klinisk genetik (creator_code:org_t)

Ingår i:Human Genetics: Springer Science and Business Media LLC101:3, s. 8-2950340-67171432-1203

Av författaren/redakt...: Broberg, K; Limon, J; Pålsson, E; Lindstrand, A; Toksvig-Larsen, ...; Mandahl, N; visa fler...; Mertens, F; visa färre...

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Reumatologi och ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.