Applications of protein-based capacitive biosensors for the detection of heavy-metal ions

• Three different methods of using protein-based capacitive biosensors for the detection of heavy-metal ions are presented. The metal-binding proteins SmtA, S100A12, MerP and four modified MerPs were immobilised as the bio-recognition element on self-assembled monolayer-modified gold electrodes. Capacitance was measured using potential square step or electrical impedance spectroscopy. The protein-metal interaction generated changes in capacitance mainly due to a protein conformational change.

Ämnesord

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Nyckelord

electrical impedance spectroscopy

self-assembled monolayer-modified gold electrodes

bio-recognition element

metal-binding proteins

protein-based capacitive biosensors

heavy-metal ion detection

electrochemical capacitance

environmental application

biomedical application

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