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  • Torres-Garcia, LauraLund University,Lunds universitet,Experimentell demensforskning,Forskargrupper vid Lunds universitet,Neural plasticitet och reparation,Experimental Dementia Research,Lund University Research Groups,Neural Plasticity and Repair (author)

Monitoring the interactions between alpha-synuclein and Tau in vitro and in vivo using bimolecular fluorescence complementation

  • Article/chapterEnglish2022

Publisher, publication year, extent ...

  • 2022-02-22
  • Springer Science and Business Media LLC,2022

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:632a07b8-3f33-4b73-b2ca-b11c480f3093
  • https://lup.lub.lu.se/record/632a07b8-3f33-4b73-b2ca-b11c480f3093URI
  • https://doi.org/10.1038/s41598-022-06846-9DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Parkinson’s disease (PD) and Alzheimer’s disease (AD) are characterized by pathological accumulation and aggregation of different amyloidogenic proteins, α-synuclein (aSyn) in PD, and amyloid-β (Aβ) and Tau in AD. Strikingly, few PD and AD patients’ brains exhibit pure pathology with most cases presenting mixed types of protein deposits in the brain. Bimolecular fluorescence complementation (BiFC) is a technique based on the complementation of two halves of a fluorescent protein, which allows direct visualization of protein–protein interactions. In the present study, we assessed the ability of aSyn and Tau to interact with each other. For in vitro evaluation, HEK293 and human neuroblastoma cells were used, while in vivo studies were performed by AAV6 injection in the substantia nigra pars compacta (SNpc) of mice and rats. We observed that the co-expression of aSyn and Tau led to the emergence of fluorescence, reflecting the interaction of the proteins in cell lines, as well as in mouse and rat SNpc. Thus, our data indicates that aSyn and Tau are able to interact with each other in a biologically relevant context, and that the BiFC assay is an effective tool for studying aSyn-Tau interactions in vitro and in different rodent models in vivo.

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  • Domingues, Joana M. P.Lund University (author)
  • Brandi, EdoardoLund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups(Swepub:lu)ed2621br (author)
  • Haikal, CarolineLund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups(Swepub:lu)ca6087ha (author)
  • Mudannayake, Janitha M.Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)ja6032mu (author)
  • Brás, Inês C.University Medical Center Göttingen (author)
  • Gerhardt, EllenUniversity Medical Center Göttingen (author)
  • Li, WenLund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups,China Medical University, Shenyang(Swepub:lu)med-wnl (author)
  • Svanbergsson, AlexanderLund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups(Swepub:lu)med-as29 (author)
  • Outeiro, Tiago F.Max Planck Institute of Experimental Medicine,University of Newcastle upon Tyne,University Medical Center Göttingen (author)
  • Gouras, Gunnar K.Lund University,Lunds universitet,Experimentell demensforskning,Forskargrupper vid Lunds universitet,Experimental Dementia Research,Lund University Research Groups(Swepub:lu)med-grg (author)
  • Li, Jia YiLund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups,China Medical University, Shenyang(Swepub:lu)mphy-jli (author)
  • Experimentell demensforskningForskargrupper vid Lunds universitet (creator_code:org_t)

Related titles

  • In:Scientific Reports: Springer Science and Business Media LLC12, s. 1-112045-2322

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