An Improvement on MS-based Epigenetic Analysis of Large Histone-derived Peptides by Using the IVU Interface.

Highly protonated histone-derived peptides impede a sufficient mass spectrometry (MS) -based epigenetic analysis since their relatively low m/z due to a high degree of proton addition to peptides would difficult to analyze the resulting complex MS/MS spectra. In order to reduce the degree of protonations, we have developed a new interface, the IVU, in which peptides are ionized under a vaporized organic solvent, and it has demonstrated that the doubly-charged histone-tail H2B peptide, PEPAKSAPAPKKGSKKAVTKAQKK (m/z 1238.243 , +2), has been detected by using the IVU interface and sequenced, which was not detectable before.

Hike, Hiroshi (author)
Usui, Fumihiko (author)
Bando, Yasuhiko (author)
Nishimura, ToshideLund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,Biosys Technologies, Inc(Swepub:lu)med-tnu (author)
Kodama, Tatsuhiko (author)
Kawamura, Takeshi (author)
TumörmikromiljöSektion I (creator_code:org_t)

In:Analytical Biochemistry: Elsevier BV486:Online 05 June 2015, s. 14-161096-03090003-2697

By the author/editor: Fukuda, Tetsuya; Hike, Hiroshi; Usui, Fumihiko; Bando, Yasuhiko; Nishimura, Toshi ...; Kodama, Tatsuhik ...; show more...; Kawamura, Takesh ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Analytical Chemi ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Medicinal Chemis ...

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