Search: onr:"swepub:oai:lup.lub.lu.se:635dfa16-c94f-4228-9e80-39735122aea3"
> (2015) >
An Improvement on M...
An Improvement on MS-based Epigenetic Analysis of Large Histone-derived Peptides by Using the IVU Interface.
-
Fukuda, Tetsuya (author)
-
Hike, Hiroshi (author)
-
Usui, Fumihiko (author)
-
show more...
-
Bando, Yasuhiko (author)
-
- Nishimura, Toshide (author)
- Lund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,Biosys Technologies, Inc
-
Kodama, Tatsuhiko (author)
-
Kawamura, Takeshi (author)
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- English.
-
In: Analytical Biochemistry. - : Elsevier BV. - 1096-0309 .- 0003-2697. ; 486:Online 05 June 2015, s. 14-16
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Highly protonated histone-derived peptides impede a sufficient mass spectrometry (MS) -based epigenetic analysis since their relatively low m/z due to a high degree of proton addition to peptides would difficult to analyze the resulting complex MS/MS spectra. In order to reduce the degree of protonations, we have developed a new interface, the IVU, in which peptides are ionized under a vaporized organic solvent, and it has demonstrated that the doubly-charged histone-tail H2B peptide, PEPAKSAPAPKKGSKKAVTKAQKK (m/z 1238.243 , +2), has been detected by using the IVU interface and sequenced, which was not detectable before.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medicinal Chemistry (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database